Anti-Phospho-Bad-S112 Rabbit Monoclonal Antibody for WB and ELISA - Q92934

Core Product Specifications and Parameters

Biological Background: BAD Function and Localization

• BAD (Bcl2-associated agonist of cell death), also known as BBC6, BCL2L8, and Bcl-xL/Bcl-2-associated death promoter, is a pro-apoptotic member of the Bcl-2 protein family.
• It promotes apoptosis by competitively binding to anti-apoptotic proteins Bcl-X(L), Bcl-2, and Bcl-W, thereby releasing pro-apoptotic factors like BAX.
• Serves as a critical mediator linking growth factor receptor signaling to the mitochondrial apoptotic pathway.
• Subcellular localization is dynamic: it shuttles between the cytoplasm and the mitochondrial outer membrane, regulated by phosphorylation.
• Phosphorylation at serine 112 (and other residues) inactivates its pro-apoptotic function by promoting 14-3-3 binding and cytoplasmic retention.
• Post-translational modifications include acetylation, methylation, and phosphorylation, as documented in the UniProt record (keywords: Phosphoprotein, Acetylation, Methylation).
• Expressed in a wide variety of tissues.
• UniProt keywords include 3D-structure, Apoptosis, Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane, and Reference proteome.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide corresponding to serine 112 of human BAD. For WB, ensure that cell lysates are prepared with phosphatase inhibitors to preserve phosphorylation; consider using a low-voltage electrotransfer to maintain phospho-epitope integrity.
• For ELISA, pair this antibody with a total BAD capture antibody to quantify phospho-specific binding; validate using peptides or treated cell extracts.
• As a rabbit monoclonal, it offers batch-to-batch consistency; nonetheless, each new lot should be titrated in your specific assay conditions.
• Cross-reacts with human and mouse BAD; for other species, sequence alignment of the epitope region is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop sourcing partner for IVD raw materials, from validated antibodies like this Phospho-Bad (Ser112) rabbit mAb to bulk reagent supply. Supporting apoptosis research and diagnostic development, we streamline procurement with expert technical consulting to accelerate your journey from concept to clinic.