Anti-Phospho-B-Raf (T401) Rabbit Monoclonal Antibody for WB, ELISA - P15056

Core Product Specifications and Parameters

Biological Background: B-Raf Function and Localization

• B-Raf is a serine/threonine-protein kinase belonging to the RAF family, encoded by the BRAF gene, and known as proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1.
• The kinase is a key component of the MAP kinase/ERK signaling pathway, transducing mitogenic signals from the cell membrane to the nucleus. It phosphorylates and activates MAP2K1 (MEK1), leading to downstream signaling. Related references: PMID:21441910, PMID:29433126
• Additionally, B-Raf phosphorylates PFKFB2, linking glycolysis regulation to proliferative signals. Related references: PMID:36402789
• May play a role in the postsynaptic responses of hippocampal neurons. Related references: PMID:1508179
• B-Raf is located in the nucleus, cytoplasm, and cell membrane.
• It is predominantly expressed in brain and testis.
• The protein undergoes various post-translational modifications including phosphorylation, acetylation, methylation, and ubiquitination. It contains a zinc-finger motif and binds ATP.
• Mutations in BRAF are associated with diseases such as cardiomyopathies, deafness, ectodermal dysplasia, and intellectual disability; it is frequently mutated in cancers (chromosomal rearrangement, proto-oncogene).

Experimental Guidance and Technical Tips

• When performing western blotting, consider including phosphatase inhibitors in lysis buffers to preserve phosphorylation status.
• To confirm specificity, it may be useful to compare signals with non-phosphorylated peptide or use phosphatase treatment.
• For ELISA applications, the antibody may be suitable for detecting phosphorylated B-Raf in cell lysates; validate with the relevant sample system.
• Given cross-reactivity with rat, this antibody could be considered for studies in rat models, but validation in your specific experimental context is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive selection of IVD raw materials for oncology and signal transduction research, including validated antibody pairs, optimized monoclonal antibodies, and ancillary reagents for kinase signaling studies. Beyond this anti-phospho-B-Raf antibody, CamelBio can source rare-target materials and provide technical consulting to support your diagnostic development from concept to clinic.