Anti-Phospho-Axl (Y702) Rabbit Monoclonal Antibody for WB, ELISA - P30530

Core Product Specifications and Parameters

Biological Background: Axl Function and Localization

• Tyrosine-protein kinase receptor UFO, also known as AXL oncogene, is a receptor tyrosine kinase that transduces signals from the extracellular matrix upon binding growth factor GAS6, regulating cell survival, proliferation, migration, and differentiation.
• Ligand-induced dimerization and autophosphorylation lead to phosphorylation of downstream substrates such as PI3K subunits, GRB2, PLCG1, and activation of AKT kinase, integrating multiple signaling cascades.
• Acts as a receptor for various viruses, including Lassa virus, lymphocytic choriomeningitis virus, and Ebolavirus, through GAS6-mediated binding to phosphatidylserine on the virion envelope.
• Promotes Zika virus entry and potentiates AXL kinase activity to antagonize type I interferon signaling via an SOCS1-dependent mechanism. Related references: PMID:28076778 PMID:29379210 PMID:31311882
• Plays an inhibitory role in TLR-mediated innate immune responses, highlighting its complex involvement in immunity.
• Highly expressed in metastatic colon tumors, with weaker expression in primary tumors and normal colon tissue, suggesting a role in cancer progression.
• Subcellular localization is predominantly at the cell membrane as a single-pass transmembrane protein.
• Characterized by immunoglobulin-like domains, protein kinase activity, and post-translational modifications including phosphorylation and glycosylation.

Experimental Guidance and Technical Tips

• The antibody was generated against a synthetic phosphopeptide corresponding to phosphorylated Y702 of human Axl; it specifically recognizes the phosphorylated form and should not cross-react with non-phosphorylated Axl.
• For WB and ELISA applications, consider using phosphatase inhibitors during sample preparation to preserve the phosphorylation signal.
• Validate the antibody's phospho-specificity by treating samples with lambda phosphatase or by comparing signals from stimulated versus unstimulated cells.
• Refer to standard protocols for phospho-antibody detection, and optimize antibody concentration and incubation conditions for your specific experimental model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutions comprehensive access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and ancillary reagents for kinase signaling and cancer research. Whether you need bulk quantities or assistance with rare target sourcing, our supply chain and technical support can help bridge the gap from concept to clinic. Explore our phospho-Axl-Y702 rabbit mAb and related products to accelerate your project development.