Anti-Phospho-ATM (S1981) Rabbit Polyclonal Antibody for WB and ELISA - Q13315

Core Product Specifications and Parameters

Biological Background: ATM Function and Localization

• ATM is a serine/threonine protein kinase that acts as a key DNA damage sensor, activating checkpoint signaling in response to double-strand breaks (DSBs) and genotoxic stresses (PMID:10550055, PMID:10839545, PMID:10910365).
  Related references:
  PMID:10550055, PMID:10839545, PMID:10910365

• It recognizes the [ST]-Q consensus motif and phosphorylates numerous downstream targets, including p53/TP53, CHEK2, BRCA1, and others, to orchestrate DNA repair and cell cycle arrest (PMID:10550055, PMID:10766245, PMID:10802669).
  Related references:
  PMID:10550055, PMID:10766245, PMID:10802669

• A critical early step in DSB signaling is ATM-mediated phosphorylation of histone H2AX at Ser139, marking chromatin flanking break sites (By similarity).

• ATM also functions in pre-B cell allelic exclusion, T-cell development, and signal transduction, and is considered a tumor suppressor.

• The kinase localizes to the nucleus, but also to cytoplasmic vesicles, the centrosome, and peroxisomes, where it phosphorylates PEX5 to promote pexophagy in response to reactive oxygen species (ROS) (PMID:26344566).
  Related references:
  PMID:26344566

• ATM phosphorylates ERCC6 to facilitate chromatin remodeling at DNA double-strand breaks (PMID:29203878), and phosphorylates TTC5/STRAP at Ser203 to drive its nuclear translocation after DNA damage (PMID:15448695).
  Related references:
  PMID:29203878, PMID:15448695

• ATM phosphorylation of DYRK2 at nuclear sites protects DYRK2 from MDM2-mediated ubiquitination and proteasomal degradation following genotoxic stress (PMID:19965871).
  Related references:
  PMID:19965871

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic phosphopeptide corresponding to the S1981 phosphorylation site of human ATM; specificity is expected for the phosphorylated form only.
• For Western blotting, include phosphatase inhibitors in lysis and wash buffers to preserve phosphorylation. Consider verifying phospho-specificity by treating samples with lambda protein phosphatase or by performing peptide blocking experiments.
• Due to the large size of ATM (~351 kDa), use a low-percentage acrylamide gel (e.g., 6%) and extended transfer times. A gradient gel (4-15%) may also improve separation.
• Validate the antibody in your specific model system; cross-reactivity with mouse is reported, but performance in other species should be tested individually.
• For ELISA, confirm coating and detection conditions, as phospho-specific antibodies may require optimized buffer pH and blocking reagents.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive supplier for IVD raw materials, offering validated antibody pairs, monoclonal/polyclonal antibodies, recombinant proteins, and ancillary reagents for DNA damage and kinase research. This anti-phospho-ATM antibody is one of many phospho-specific tools in our portfolio, and we can assist with sourcing rare targets or custom development needs. From early-stage R&D to clinical assay manufacturing, CamelBio provides the materials and technical support to streamline your project.