Anti-Phospho-ALOX5-S271 Polyclonal Antibody for WB, ELISA - P09917

Core Product Specifications and Parameters

Biological Background: ALOX5 Function and Localization

• Catalyzes the first two steps in leukotriene biosynthesis from arachidonate, producing potent pro-inflammatory mediators. Related references: PMID:19022417, PMID:21233389, PMID:22516296
• Functions as a lipoxin synthase converting (15S)-HETE into a conjugate tetraene, contributing to anti-inflammatory responses. Related references: PMID:31664810
• Oxygenates eicosapentaenoate (EPA) and docosahexaenoate (DHA) to form specialized pro-resolving mediators (SPMs) such as resolvins, participating in inflammation resolution. Related references: PMID:17114001, PMID:21206090, PMID:31664810
• Also catalyzes oxygenation of arachidonate into 8-HPETE and 12-HPETE. Related references: PMID:23246375
• Involved in adaptive immunity by regulating B cells and follicular helper T cells and interacts with PIK3R1 to bridge ALOX5 with CD40 for ROS production. Related references: PMID:21200133
• Subcellularly located in cytoplasm, nucleus matrix, nuclear membrane, perinuclear region, cytosol, nuclear envelope, and nucleus intermembrane space.
• Key phosphorylation site at serine 271 may modulate enzymatic activity or localization.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around S271; the antibody is expected to specifically recognize the phosphorylated form of human ALOX5.
• Use phosphatase inhibitors during sample preparation to preserve phosphorylation.
• For Western blot, a band near 78 kDa is anticipated; validate in stimulated cell or tissue models known to activate ALOX5.
• This antibody is intended for human samples only; cross-reactivity with other species has not been determined.
• Optimize working dilutions for WB and ELISA in your experimental system; typical starting points should be determined empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with one-stop access to IVD raw materials, supporting projects in leukotriene signaling and inflammation research. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to streamline your development pipeline. Partner with us for reliable, scalable supply solutions from concept to clinic.