Anti-Phospho-AKT1 (Thr450) Rabbit Polyclonal Antibody for WB, ELISA - P31749

Core Product Specifications and Parameters

Biological Background: AKT1 Function and Localization

• AKT1 is the founding member of the AKT serine/threonine kinase family, which also includes AKT2 and AKT3, and governs fundamental processes such as metabolism, proliferation, survival, growth, and angiogenesis (PubMed:11882383, PubMed:15526160, PubMed:15861136). Related references: PMID:11882383, PMID:15526160, PMID:15861136
• In the insulin signaling cascade, AKT1 phosphorylates TBC1D4 to promote GLUT4 translocation and glucose uptake (PubMed:11994271) and inhibits GSK3 to drive glycogen synthesis. Related references: PMID:11994271
• AKT1 supports cell survival by directly phosphorylating and inactivating pro-apoptotic proteins such as MAP3K5/ASK1 (PubMed:11154276) and BAD (PubMed:10926925), and by causing cytoplasmic retention of FOXO transcription factors (PubMed:10358075). Related references: PMID:11154276, PMID:10926925, PMID:10358075
• Through TSC2 phosphorylation, AKT1 activates mTORC1, coupling growth factor signals to protein synthesis, ribosome biogenesis, and cell growth (PubMed:12150915, PubMed:12172553). Related references: PMID:12150915, PMID:12172553
• AKT1 shuttles between the cytoplasm, nucleus, plasma membrane, and mitochondrial intermembrane space; this dynamic localization is essential for accessing distinct downstream targets.
• Widely expressed in human tissues, AKT1 protein levels and activating phosphorylations are frequently elevated in cancers, including prostate and breast malignancies, where they correlate with tumor progression.
• AKT1 is subject to extensive post-translational regulation: phosphorylation at Thr308 and Ser473 is required for full kinase activity, while acetylation, ubiquitination, and glycosylation further modulate its stability and function.

Experimental Guidance and Technical Tips

• For Western blot (WB), prepare cell or tissue lysates with phosphatase inhibitors to preserve the phospho-Thr450 signal; a specific band near 56 kDa is expected in human and mouse samples.
• In ELISA applications, a phospho-peptide matching the immunogen may be used as a coating antigen to capture the antibody; users should optimize coating concentrations and antibody dilutions for their specific assay.
• Because polyclonal antibodies can exhibit lot-to-lot variability, inclusion of appropriate positive and negative controls (e.g., phosphatase-treated lysates) is recommended.
• This antibody is intended for research use in detecting AKT1 phosphorylation; validation in the end-user’s sample matrix and assay system is essential for reliable results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with premium IVD raw materials, exemplified by this phospho-AKT1 (Thr450) rabbit polyclonal antibody, a targeted tool for studying AKT-driven signaling in cancer and metabolic disease. Our comprehensive portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and expert sourcing support for rare-target raw materials, all designed to streamline your assay development from concept to clinical application.