Anti-Phospho-AKT1 (T450) Rabbit Monoclonal Antibody for WB, ELISA - P31749

Core Product Specifications and Parameters

Biological Background: AKT1 Function and Localization

• AKT1 is a serine/threonine-protein kinase, one of three closely related AKT isoforms (AKT1, AKT2, AKT3), also known as Protein Kinase B (PKB), that regulate diverse cellular processes including metabolism, proliferation, survival, growth, and angiogenesis. Related references: PMID:11882383, PMID:15526160, PMID:15861136
• AKT1 mediates signaling downstream of phosphoinositide 3-kinase (PI3K) in response to growth factors such as insulin, IGF1, EGF, and PDGF, and phosphorylates a broad range of substrates. Related references: PMID:12176338, PMID:12964941
• Phosphorylation of TSC2 at Ser-939 and Thr-1462 by AKT1 leads to mTORC1 pathway activation, promoting protein synthesis and cell growth. Related references: PMID:12150915, PMID:12172553
• AKT1 regulates cell survival by phosphorylating and inhibiting pro-apoptotic factors such as MAP3K5 (ASK1) and BAD, and by activating survival transcription factors like CREB1. Related references: PMID:11154276, PMID:10926925, PMID:9829964
• AKT1 regulates insulin-stimulated glucose transport by phosphorylating TBC1D4, which triggers binding to 14-3-3 proteins and facilitates GLUT4 translocation to the cell surface. Related references: PMID:11994271
• AKT1 shuttles between the cytoplasm, nucleus, cell membrane, and mitochondrial intermembrane space, with phosphorylation at Thr450 (and other sites) influencing its activation and localization.
• The kinase is expressed ubiquitously in human tissues, with increased protein levels and phosphorylation observed in prostate and breast cancers, highlighting its role as a proto-oncogene.
• Key post-translational modifications include phosphorylation, acetylation, and ubiquitination, which modulate its activity, stability, and interactions.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide corresponding to residues surrounding Thr450 of human AKT1. Ensure that your experimental conditions preserve protein phosphorylation; consider using phosphatase inhibitors during sample preparation.
• For Western blot (WB), the antibody is designed to specifically recognize phosphorylated AKT1 at Thr450. Validate the signal specificity using phosphatase-treated lysates or a non-phosphorylated peptide blocking control if needed.
• The antibody is suitable for ELISA applications. Consider optimizing coating and detection conditions using purified phosphorylated AKT1 protein or peptide.
• Cross-reactivity has been demonstrated with human and rat AKT1. Verify reactivity with other species if required, as AKT1 T450 is highly conserved but may have slight variations.
• Given AKT1's role in multiple signaling pathways, use appropriate stimuli (e.g., insulin, growth factors) to induce phosphorylation at Thr450 in your experimental model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and institutes with a one-stop sourcing solution for high-quality IVD raw materials. This anti-phospho-AKT1 (T450) antibody is particularly valuable for researchers investigating AKT signaling in cancer, diabetes, and cell survival pathways, and CamelBio can support your projects with optimized antibody pairs, recombinant proteins, and ancillary reagents. Whether you need bulk quantities, custom formulations, or assistance in sourcing rare targets, our team is ready to accelerate your development from concept to clinic.