Anti-Phospho-AKT1-T308 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P31749

Core Product Specifications and Parameters

Biological Background: AKT1 Function and Localization

• AKT1 is a member of the AKT serine/threonine kinase family (AKT1, AKT2, AKT3) that regulates numerous cellular processes including metabolism, proliferation, cell survival, growth and angiogenesis. Related references: PMID:11882383 PMID:15526160 PMID:15861136
• Functions downstream of PI3K to mediate the effects of insulin, IGF1 and growth factors such as PDGF and EGF. Related references: PMID:12176338 PMID:12964941
• Phosphorylates TSC2 at Ser-939 and Thr-1462, leading to mTORC1 activation and enhanced protein synthesis. Related references: PMID:12150915 PMID:12172553
• Phosphorylates and inhibits MAP3K5, promoting cell survival under oxidative stress conditions. Related references: PMID:11154276
• Phosphorylates FOXO transcription factors (e.g., FOXO1 at Thr-24, Ser-256, Ser-319), leading to their cytoplasmic retention and suppression of pro-apoptotic gene expression. Related references: PMID:10358075
• Phosphorylates BAD, thereby suppressing its pro-apoptotic activity. Related references: PMID:10926925
• Localizes to the cytoplasm, nucleus, cell membrane and mitochondrial intermembrane space, enabling diverse signaling roles.
• Widely expressed in human tissues; elevated protein levels are observed in prostate cancer and breast cancer progression.

Experimental Guidance and Technical Tips

• The immunogen targets the phosphorylation site at Thr-308, a critical activation loop residue in AKT1. For total AKT1 detection, consider a separate pan‑AKT antibody.
• In WB, the expected band migrates around 56 kDa. Validate phosphorylation status by treating cells with stimuli such as insulin or growth factors; include phosphatase inhibitors during lysis.
• For IHC-P, antigen retrieval may be necessary. Use phospho‑peptide blocking and tissue sections with known AKT1 activation status as controls.
• ELISA applications should be optimized by checkerboard titration; confirm specificity with phosphorylated and non‑phosphorylated peptides.
• Cross‑reactivity with mouse and rat AKT1 is reported; verify sequence homology at the epitope if studying other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one‑stop access to IVD raw materials, technical services and consulting—from concept to clinic. This anti‑phospho‑AKT1‑T308 antibody supports research into PI3K/AKT/mTOR signaling, cancer biology and metabolic disorders. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents and customized sourcing for rare‑target raw materials—helping you accelerate assay development and secure a reliable supply chain.