Anti-Phospho-AKT1 (Ser473) Rabbit Polyclonal Antibody for WB, ELISA - P31749

Core Product Specifications and Parameters

Biological Background: AKT1 Function and Localization

• AKT1 (RAC-alpha serine/threonine-protein kinase, also known as PKBα) is a serine/threonine-protein kinase belonging to the AKT family, which in humans comprises three closely related isoforms (AKT1, AKT2, AKT3) that control metabolism, proliferation, survival, growth, and angiogenesis. Related references: PMID:11882383, PMID:15526160, PMID:15861136

• It operates downstream of PI3K and transduces signals from insulin, IGF1, EGF, and PDGF; it phosphorylates over 100 downstream targets, including GSK3, TSC2, FOXO transcription factors, and mTORC1 pathway components, thereby coordinating glucose metabolism, protein synthesis, and gene expression. Related references: PMID:12176338, PMID:12964941, PMID:11882383, PMID:15526160, PMID:21432781

• Phosphorylation of TSC2 at Ser939 and Thr1462 by AKT1 relieves mTORC1 inhibition, driving cell growth and protein synthesis; conversely, AKT1 phosphorylates and inhibits GSK3α/β to promote glycogen synthesis and cell cycle progression. Related references: PMID:12150915, PMID:12172553

• AKT1 promotes cell survival by phosphorylating and inactivating pro-apoptotic proteins such as BAD (Ser136) and MAP3K5/ASK1 (Ser83), and by activating CREB1 to upregulate anti-apoptotic BCL2 and MCL1. Related references: PMID:10926925, PMID:11154276, PMID:9829964

• It regulates cytoskeletal dynamics and cell migration by phosphorylating substrates such as palladin (PALLD) and SPATA13, influencing adhesion turnover and motility. Related references: PMID:20471940, PMID:19934221

• AKT1 localizes to the cytoplasm, nucleus, plasma membrane, and mitochondrial intermembrane space. It is ubiquitously expressed, with elevated phospho-Ser473 levels observed in prostate and breast cancers, linking it to oncogenic signaling.

Experimental Guidance and Technical Tips

• The antibody is raised against a synthetic phosphorylated peptide corresponding to residues surrounding Ser473 of human Akt1; consider using phosphatase-treated lysates or a non-phospho-AKT1 antibody as a negative control to verify phosphorylation specificity.
• In WB, expect a single band at ~56 kDa; use appropriate positive controls (e.g., lysates from insulin- or EGF-stimulated cells) and optimize loading amounts and exposure times.
• For ELISA, plate coating with phospho-peptide and non-phospho-peptide can help assess phospho-specific binding; include blocking and washing steps to reduce nonspecific background.
• Cross-reactivity is indicated for human, mouse, and rat; however, validation in each species' relevant sample matrix is recommended.
• Since the antibody recognizes the phosphorylated form of AKT1, it may also detect other AKT isoforms (AKT2, AKT3) if the epitope is conserved; confirm specificity with isoform-specific knockdown or knockout models if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw material procurement, offering a comprehensive portfolio that includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare targets. This Phospho-AKT1 (Ser473) antibody supports research into AKT signaling pathways, particularly in cancer, metabolic disorders, and cell survival studies. From concept to clinic, CamelBio streamlines access to critical components, technical expertise, and regulatory consulting to accelerate diagnostic development.