Anti-Phospho-AKT1-S473 Monoclonal Antibody for WB, IHC-P, ELISA - P31749

Core Product Specifications and Parameters

Biological Background: RAC-alpha serine/threonine-protein kinase (AKT1) Function and Localization

• AKT1 is a serine/threonine-protein kinase that regulates metabolism, proliferation, cell survival, growth, and angiogenesis through phosphorylation of downstream substrates. Related references: PMID:11882383, PMID:15526160, PMID:15861136
• It phosphorylates TBC1D4 to promote insulin-stimulated glucose transport. Related references: PMID:11994271
• Through phosphorylation of TSC2, AKT1 activates the mTORC1 signaling pathway, enhancing protein synthesis. Related references: PMID:12150915, PMID:12172553
• AKT1 promotes cell survival by phosphorylating MAP3K5/ASK1 at Ser-83 to inhibit oxidative stress-induced apoptosis. Related references: PMID:11154276
• It phosphorylates FOXO transcription factors (FOXO1, FOXO3, FOXO4) to exclude them from the nucleus, suppressing pro-apoptotic gene expression. Related references: PMID:10358075
• Subcellular localization includes cytoplasm, nucleus, cell membrane, and mitochondrial intermembrane space, reflecting its diverse signaling roles.
• AKT1 is expressed in all human cell types, with elevated levels in prostate and breast cancers, and the phospho-Ser473 form is linked to cancer progression.
• Key post-translational modifications include phosphorylation at Ser473 and Thr308 for full activation, as well as acetylation and glycosylation.

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic phosphopeptide around Ser473 of human Akt1. The immunogen design targets the activated form of Akt1; non-phosphorylated Akt1 may not be detected.
• For Western blot (WB), consider using phosphatase inhibitors during sample preparation to preserve phosphorylation states. The predicted molecular weight is 56 kDa (P31749).
• For immunohistochemistry (IHC-P), antigen retrieval conditions and tissue fixation may influence phospho-epitope detection; validate in known positive control tissues (e.g., cancer specimens).
• For ELISA, ensure the coating conditions preserve the phospho-epitope; the antibody may be used in combination with a total Akt1 detection antibody for phospho/total ratio analysis.
• Cross-reactivity with mouse and rat Akt1 is expected based on sequence homology; differences in phospho-site accessibility may require species-specific validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research labs a streamlined sourcing solution for IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our portfolio covers key signaling targets like phospho-AKT1 (S473) for oncology and metabolism research applications. From concept to clinic, CamelBio provides the supply chain reliability and technical support to accelerate your assay development.