Anti-Phospho-Akt-T308 Rabbit Monoclonal Antibody for WB, ELISA - P31749 / P31751 / Q9Y243

Core Product Specifications and Parameters

Biological Background: AKT (AKT1/AKT2/AKT3) Function and Localization

• AKT1 is one of three closely related serine/threonine-protein kinases (AKT1, AKT2, AKT3) that regulate critical processes such as metabolism, proliferation, cell survival, growth, and angiogenesis. Related references: PMID:11882383, PMID:15526160, PMID:15861136

• As a central node in PI3K signaling, AKT phosphorylates over 100 downstream substrates on serine/threonine residues to mediate effects of insulin, growth factors, and other stimuli. Related references: PMID:11882383, PMID:15526160, PMID:21432781

• In glucose homeostasis, AKT promotes insulin-stimulated glucose uptake via SLC2A4/GLUT4 translocation and glycogen synthesis by inhibiting GSK3A/B; it also phosphorylates TBC1D4 to trigger 14-3-3 binding essential for glucose transport. Related references: PMID:11994271

• AKT drives cell survival by phosphorylating and inhibiting pro-apoptotic substrates such as MAP3K5, BAD, and FOXO transcription factors, which leads to cytoplasmic sequestration of FOXO proteins and upregulation of anti-apoptotic genes like BCL2 and MCL1 via CREB1. Related references: PMID:11154276, PMID:10358075, PMID:9829964, PMID:10926925

• AKT activates mTORC1 signaling by phosphorylating TSC2 and other regulators (CASTOR1, DEPDC5), thereby controlling protein synthesis and cell growth. Related references: PMID:12150915, PMID:12172553, PMID:31548394, PMID:33594058

• Subcellularly, AKT is found in the cytoplasm, nucleus, cell membrane, and mitochondrial intermembrane space, reflecting its diverse functions.

• In humans, AKT1 is ubiquitously expressed, with elevated phosphorylated forms observed in prostate and breast cancers, suggesting roles in tumor progression.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phospho-peptide around Thr308 of human Akt; phosphorylation at this residue is critical for Akt activation. Therefore, the antibody is specific for the phosphorylated form and should not recognize the non-phosphorylated Akt.
• Because Thr308 is conserved across AKT1, AKT2, and AKT3 isoforms, the antibody is expected to cross-react with the corresponding phosphorylated forms in mouse and rat, as indicated by cross-reactivity data.
• For Western blot analysis, use appropriate phosphatase inhibitors during sample preparation to preserve phosphorylation. The predicted molecular weights of the isoforms are ~48-55 kDa; confirm band specificity with positive and negative controls (e.g., insulin-stimulated vs. untreated cells).
• ELISA applications may be suitable for capturing or detecting phospho-Akt in cell lysates; optimize coating and detection conditions for your specific matrix.
• Given that T308 phosphorylation is dynamic, include proper experimental controls for assessing signaling pathway activation (e.g., PI3K inhibitor treatment).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted partner for diagnostic manufacturers and research labs, offering a comprehensive range of IVD raw materials including phospho-specific antibodies like this anti-Phospho-Akt-T308 antibody. Our portfolio covers rare targets, bulk ancillary reagents, and validated antibody pairs to support your assay development pipeline. From single reagents to complete sourcing solutions, we help streamline your journey from concept to clinic.