Anti-Phospho-Acetyl CoA Carboxylase 1 (S79) Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - Q13085

Core Product Specifications and Parameters

Biological Background: Acetyl-CoA Carboxylase 1 (ACC1) Function and Localization

• Acetyl-CoA carboxylase 1 (ACC1) is encoded by the ACACA gene, also known as ACC1 or ACCA, and is the rate-limiting enzyme in de novo fatty acid biosynthesis.
• It catalyzes the ATP-dependent carboxylation of acetyl-CoA to malonyl-CoA in a two-step reaction involving a biotin carboxyl carrier domain (PubMed:20457939, PubMed:20952656, PubMed:29899443). Related references: PMID:20457939 PMID:20952656 PMID:29899443
• The enzyme localizes to the cytosol.
• Tissue expression is observed in brain, placenta, skeletal muscle, renal, pancreatic, and adipose tissues; low expression in lung; and no detection in liver.
• ACC1 is a biotin-dependent carboxylase with binding sites for ATP and metal ions (Mg2+, Mn2+). It is subject to post-translational modifications including phosphorylation and acetylation.
• It plays a critical role in lipid metabolism by regulating the synthesis of long-chain fatty acids essential for membrane biogenesis and energy storage.

Experimental Guidance and Technical Tips

• For Western blotting, use a low-percentage gel (e.g., 6%) to resolve the ~266 kDa ACC1 protein, and include phosphatase inhibitors during lysis to preserve phosphorylation at S79.
• Verify antibody specificity by pre-incubating the antibody with a non-phosphorylated S79 peptide to confirm phospho-dependent binding.
• In immunofluorescence or ICC, expect cytoplasmic staining; co-stain with a cytosolic marker to validate localization.
• For ELISA, the phosphorylated peptide immunogen can be used as a positive control; ensure the coating buffer is compatible with phosphopeptides.
• Validate the antibody in relevant cell lines that express ACC1 (e.g., HepG2, 3T3-L1) with and without AMPK activation to test phosphorylation-inducible detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials, supporting diagnostic developers, labs, and research institutes from concept to clinic. For lipid metabolism and metabolic disease research, we provide validated antibody pairs, bulk ancillary reagents, and rare target sourcing including phospho-specific antibodies like this anti-Phospho-ACC1 (S79) mAb. Our extensive supply chain ensures timely delivery of high-quality materials tailored to your assay development needs.