Anti-PHLPP2 Polyclonal Antibody for WB, IF/ICC, ELISA - Q6ZVD8

Core Product Specifications and Parameters

Biological Background: PHLPP2 Function and Localization

• PHLPP2 (PH domain leucine-rich repeat-containing protein phosphatase 2), also known as PHLPPL or PPM3B, is a member of the PHLPP family of phosphatases that regulate AGC kinase signaling.
• It dephosphorylates key serine residues in the hydrophobic motif of AGC kinases, including Akt1 (Ser-473), PRKCB (Ser-660), and PRKCA (Ser-657), thereby inactivating these kinases and promoting apoptosis or reducing proliferation.
• PHLPP2 functions as a tumor suppressor, and its expression is downregulated in many cancers, including colorectal cancer where it is lost in about 80% of tumors (at protein level).
• Additionally, PHLPP2 dephosphorylates and activates STK4 (MST1) at Thr-387, leading to apoptosis. Related references: PMID:20513427
• It dephosphorylates RPS6KB1 (S6K1), regulating cap-dependent translation. Related references: PMID:21986499
• PHLPP2 also dephosphorylates RAF1, inhibiting its kinase activity and thereby modulating the MAPK/ERK pathway. Related references: PMID:24530606
• Subcellularly, PHLPP2 localizes to the cytoplasm, membrane, and nucleus, consistent with its roles in various signaling compartments.
• The protein contains leucine-rich repeats and a PH domain, which are important for protein-protein interactions and membrane targeting.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (amino acids 1250-1323) of human PHLPP2; consider using a blocking peptide to confirm antibody specificity in relevant assays.
• For Western blotting (WB), the predicted molecular weight of PHLPP2 is ~147 kDa; use an appropriate gel percentage (e.g., 6-8% SDS-PAGE) and ensure adequate transfer conditions for high-molecular-weight proteins.
• In immunofluorescence/immunocytochemistry (IF/ICC), PHLPP2 exhibits cytoplasmic, membrane, and nuclear localization; optimize fixation and permeabilization protocols for each compartment.
• For ELISA, titrate the antibody against the immunogen peptide or recombinant protein to determine optimal working concentration for detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. For research on tumor suppressors and kinase signaling like PHLPP2, CamelBio can support your assay development and raw material procurement needs.