Anti-PHKG2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P15735

Core Product Specifications and Parameters

Biological Background: PHKG2 Function and Localization

• PHKG2 encodes the catalytic gamma subunit of phosphorylase b kinase, acting as a serine/threonine-protein kinase and transferase.
• It mediates neural and hormonal regulation of glycogenolysis by phosphorylating and activating glycogen phosphorylase.
• In vitro, PHKG2 phosphorylates glycogen phosphorylase, muscle form (PYGM), confirming its direct enzymatic role. Related references: PMID:35549678
• The protein contains an ATP-binding site and a calmodulin-binding domain, linking its activity to calcium signaling.
• PHKG2 is subject to phosphorylation and alternative splicing, generating multiple isoforms with potential functional diversity.
• Mutations in PHKG2 are associated with glycogen storage disease type IXc (GSD9C), as reflected in disease variant annotations.
• Additional UniProt keywords include 3D-structure, proteomics identification, and reference proteome, indicating comprehensive characterization.

Experimental Guidance and Technical Tips

• For Western blotting, consider using a loading control with a molecular weight near 46 kDa.
• Validate antibody specificity with appropriate positive (e.g., liver or testis lysates) and negative controls.
• When performing IF/ICC, confirm subcellular localization using organelle-specific markers.
• ELISA optimization may require purified PHKG2 protein or peptide to establish a standard curve.
• Due to the polyclonal nature, slight lot-to-lot variation is possible; verify performance in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutes a comprehensive, one-stop supply channel for IVD raw materials. This anti-PHKG2 antibody supports glycogen metabolism and glycogen storage disease research, including GSD type IX studies. Beyond individual antibodies, we provide validated antibody pairs, optimized monoclonal and polyclonal reagents, bulk ancillary materials, and rare target sourcing to streamline your assay development from concept to clinic.