Anti-PHKG2 Rabbit Polyclonal Antibody for WB, ELISA - P15735

Core Product Specifications and Parameters

Biological Background: PHKG2 Function and Localization

• PHKG2 encodes the gamma-2 catalytic subunit of phosphorylase b kinase (PHK), a serine/threonine-protein kinase that plays a critical role in glycogenolysis by phosphorylating and activating glycogen phosphorylase.
• This liver/testis isoform (also known as PSK-C3 or phosphorylase kinase subunit gamma-2) is expressed in liver and testis, where it may regulate glycogen breakdown in response to neural and hormonal signals.
• In vitro, PHKG2 directly phosphorylates PYGM (glycogen phosphorylase muscle form), as demonstrated by functional assays. Related references: PMID:35549678
• The protein contains domains for ATP binding and calmodulin binding, indicating calcium/calmodulin-dependent regulation of its kinase activity.
• PHKG2 is subject to alternative splicing, and its activity is regulated by phosphorylation (as a phosphoprotein), consistent with its role in signal transduction.
• Mutations in PHKG2 are associated with glycogen storage disease type IXc (GSD9C), a metabolic disorder affecting liver glycogen metabolism.
• The canonical isoform has a calculated molecular weight of approximately 46 kDa (UniProt: 46,442 Da), aligning with the observed band in western blotting.

Experimental Guidance and Technical Tips

• The immunogen covers the C-terminal region (aa 237-406) of human PHKG2; this region may allow detection of full-length and certain alternative isoforms.
• For western blot, validate the antibody using lysates from human liver or testis tissue, or relevant cell lines; consider using a loading control such as GAPDH.
• When performing ELISA, titrate the antibody to determine the optimal working concentration for your specific assay conditions.
• Due to reported cross-reactivity with mouse and rat, the antibody may be suitable for studies in rodent models; confirm reactivity in the appropriate sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories streamlined access to IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. For targets such as PHKG2—involved in glycogen metabolism and related disorders—CamelBio can source rare-pattern raw materials to support your assay development from concept to clinic. Contact us to explore customized procurement solutions.