Anti-PHKB Polyclonal Antibody for WB, ELISA - Q93100

Core Product Specifications and Parameters

Biological Background: PHKB Function and Localization

• PHKB encodes the regulatory subunit beta of phosphorylase b kinase (PhK), a serine/threonine-specific protein kinase that phosphorylates substrates such as troponin I.
• The beta subunit acts as a regulatory unit, modulating the holoenzyme's activity in response to phosphorylation events.
• The protein is associated with the cell membrane (UniProt annotation: SUBCELLULAR LOCATION: Cell membrane).
• Post-translational modifications include acetylation, phosphorylation, and prenylation, suggesting dynamic regulation.
• Alternative splicing generates multiple isoforms, contributing to functional diversity.
• Keywords indicate involvement in carbohydrate and glycogen metabolism, and it is linked to glycogen storage disease (disease variant).
• The molecular weight is 124884 Da, consistent with the observed ~125 kDa band in applications.

Experimental Guidance and Technical Tips

• The immunogen covers the C-terminal region (aa 854-1093), which may help detect both full-length and truncated forms of PHKB.
• For Western blot, consider using a suitable positive control lysate from human or mouse tissues/cell lines expressing PHKB.
• For ELISA, the antibody may be used for coating or detection; validate with recombinant PHKB protein.
• Cross-reactivity with human and mouse suggests utility in both species; validation in the relevant sample system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our portfolio includes validated antibody pairs, optimized polyclonal antibodies like this anti-PHKB antibody, and bulk ancillary reagents for glycogen metabolism and related disease research. For rare target raw-material sourcing, CamelBio acts as your reliable bridge to accelerate development.