Anti-PHGDH Rabbit Polyclonal Antibody for WB, IF/ICC, IP, ELISA - O43175

Core Product Specifications and Parameters

Biological Background: PHGDH Function and Localization

• PHGDH (D-3-phosphoglycerate dehydrogenase) is a metabolic enzyme that catalyzes the first step in the phosphorylated L-serine biosynthesis pathway, converting 3-phospho-D-glycerate to 3-phosphonooxypyruvate.
• Additionally, PHGDH exhibits 2-oxoglutarate reductase and malate dehydrogenase activities, catalyzing the reversible oxidation of 2-hydroxyglutarate to 2-oxoglutarate and (S)-malate to oxaloacetate.
• The protein is encoded by the PHGDH gene (also known as PGDH3), located on human chromosome 1.
• Keywords associated with PHGDH include Amino-acid biosynthesis, Serine biosynthesis, NAD, Oxidoreductase, and post-translational modifications such as Acetylation, Phosphoprotein, Isopeptide bond, and Ubl conjugation.
• PHGDH has been implicated in disease variants (Disease variant keyword) and is a target of proteomics identification (Proteomics identification keyword).
• The calculated molecular weight of PHGDH is approximately 57 kDa (UniProt: 56,651 Da).

Experimental Guidance and Technical Tips

• The immunogen used for this antibody corresponds to amino acids 264-533 of human PHGDH (NP_006614.2), a region within the C-terminal domain. It is expected to recognize full-length PHGDH and possibly truncated variants containing this epitope.
• For Western blot (WB), consider using standard denaturing conditions and validate detection with positive controls such as cell lines with known PHGDH expression (e.g., HeLa, HEK293T). Confirm band specificity near the predicted molecular weight of 57 kDa.
• In immunofluorescence/immunocytochemistry (IF/ICC), optimize fixation and permeabilization protocols for reliable visualization of PHGDH localization. Include appropriate controls to assess antibody specificity.
• For immunoprecipitation (IP), verify the antibody's ability to enrich PHGDH from lysates using recommended buffers and confirm pull-down efficiency by WB.
• When using in ELISA, consider pairing with a suitable detection antibody and validate against recombinant PHGDH or endogenous targets in sample matrices.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a streamlined pathway to procuring high-quality IVD raw materials, including this PHGDH rabbit polyclonal antibody, for serine biosynthesis and metabolic disease research. Our supply chain covers validated antibody pairs, monoclonal and polyclonal antibodies in bulk, and ancillary reagents tailored to your assay development needs. For custom or rare targets like PHGDH, our sourcing experts facilitate quick access to optimized materials, supporting your project from concept to clinic.