Anti-PHF21B Rabbit Polyclonal Antibody for WB & ELISA - Q96EK2

Core Product Specifications and Parameters

Biological Background: PHD finger protein 21B Function and Localization

• PHD finger protein 21B (PHF21B) is a zinc-finger protein characterized by a plant homeodomain (PHD) finger, which typically facilitates binding to methylated histones and other chromatin-associated modifications.
• The PHF21B gene undergoes alternative splicing, generating multiple transcript variants that may exhibit isoform-specific functions.
• As a metal-binding protein, PHF21B coordinates zinc ions through its zinc-finger motifs, contributing to structural stability and functional interactions.
• Coiled-coil regions within PHF21B likely mediate protein-protein interactions, potentially linking it to multiprotein complexes.
• UniProt classifies PHF21B as a reference proteome member and notes its identification through large-scale proteomics, supporting its endogenous expression in human cells.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 322-531, covering part of the PHD finger domain; verify epitope accessibility in native versus denatured conditions.
• For Western blot, use standard lysis buffers (e.g., RIPA) and validate detection in human, mouse, and rat samples as indicated; the predicted molecular weight is ~57 kDa.
• For ELISA, consider titrating the antibody and pairing with appropriate secondary detection reagents; perform cross-species validation due to reported cross-reactivity.
• Since this is a polyclonal antibody, lot-to-lot consistency should be monitored, and blocking conditions may need optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research teams with a comprehensive portfolio of IVD raw materials, including validated antibodies, recombinant proteins, and ancillary reagents. This PHF21B polyclonal antibody serves epigenetics and chromatin research applications, and we can assist with bulk orders, customized antibody panels, and alternative clone development. Contact us to explore one-stop procurement solutions tailored to your assay development pipeline.