Anti-PHF19 Polyclonal Antibody for WB, IF/ICC, ELISA - Q5T6S3

Core Product Specifications and Parameters

Biological Background: PHF19 Function and Localization

• PHD finger protein 19 (PHF19), also known as Polycomb-like protein 3 (PCL3), MTF2L1, and TDRD19B, is a ~66 kDa polycomb group (PcG) protein encoded by the PHF19 gene (GeneID: 26147).
• PHF19 specifically recognizes histone H3 trimethylated at lysine 36 (H3K36me3) and recruits the Polycomb repressive complex 2 (PRC2), enhancing its H3K27me3 methyltransferase activity and contributing to transcriptional silencing.
  Related references: PMID:15563832, PMID:18691976, PMID:23104054
• In embryonic stem cells, PHF19 binds H3K36me3 at active genes and recruits the histone demethylases RIOX1 or KDM2B, leading to H3K36 demethylation, PRC2 recruitment, and de novo gene silencing during differentiation.
  Related references: PMID:23160351
• PHF19 also binds H3K36 dimethylation (H3K36me2) and is involved in recruiting PRC2 to CpG island promoters, supporting embryonic stem cell self-renewal.
  Related references: PMID:23104054
• Isoform 1 and isoform 2 of PHF19 act as transcriptional repressors, inhibiting transcription from an HSV-tk promoter.
  Related references: PMID:15563832
• Subcellularly, PHF19 localizes to the nucleus, consistent with its role as a chromatin regulator.
• PHF19 is widely expressed, with isoform 1 in thymus, heart, lung, and kidney, and isoform 2 enriched in placenta, skeletal muscle, and kidney; both isoforms are overexpressed in multiple cancers, including colon, lung, liver, and melanoma.
• UniProt keywords highlight alternative splicing, chromatin regulator, metal-binding, nuclear phosphoprotein, zinc-finger repeats, and transcription repression.

Experimental Guidance and Technical Tips

• The immunogen (aa 1–207) is highly conserved across human, mouse, and rat, supporting reported cross-reactivity; validate reactivity in your specific sample system before large-scale use.
• For western blotting, anticipate a band near 66 kDa; optimize gel percentages and transfer conditions, and include appropriate loading controls.
• In IF/ICC, nuclear staining is expected; use DAPI co-staining and negative controls (e.g., isotype control or no-primary control) to confirm signal specificity.
• For ELISA, titrate the antibody against recombinant immunogen or target cell lysates; note that quantitative performance metrics are not provided.
• The antibody may detect multiple isoforms; refer to UniProt Q5T6S3 for isoform-specific information.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material sourcing partner, providing diagnostic manufacturers and research labs with reliable access to antibodies, recombinant proteins, and ancillary reagents. Our anti-PHF19 polyclonal antibody supports epigenetic and cancer research applications, and we offer complementary products such as validated antibody pairs and detection systems. Contact our team to explore bulk ordering, custom development, or to source rare-target materials for your next project.