Anti-PHF11 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9UIL8

Core Product Specifications and Parameters

Biological Background: PHF11 Function and Localization

• PHF11 (PHD finger protein 11), also known as BRCA1 C-terminus-associated protein (BCAP) and renal carcinoma antigen NY-REN-34, is encoded by the PHF11 gene in humans.
• It functions as a positive regulator of Th1-type cytokine gene expression, implicating it in immune response modulation.
• The protein localizes predominantly to the nucleus, consistent with its role in transcriptional regulation.
• PHF11 contains a PHD finger domain, a zinc-finger motif that typically mediates protein-protein interactions and may bind to methylated histones.
• It is highly expressed in immune cells, particularly T and B lymphocytes, natural killer cells, and mature dendritic cells, with elevated levels in Th1 compared to Th2 cells.
• Expression is low but detectable in a range of normal tissues, including lung, testis, small intestine, breast, liver, and placenta.
• Alternative splicing of PHF11 yields multiple transcript variants, which may contribute to functional diversity.

Experimental Guidance and Technical Tips

• For Western blotting, expect a band near 38 kDa; validate using lysates from Th1-enriched cell populations.
• In IHC-P, nuclear staining may be observed in lymphoid tissues; include appropriate positive and negative controls.
• During IF/ICC, anticipate nuclear localization; optimize fixation and permeabilization conditions for antigen accessibility.
• For ELISA applications, consider the antibody as either capture or detection reagent; assess pairing with other PHF11-specific antibodies.
• Due to the polyclonal nature, cross-reactivity with other PHD finger-containing proteins cannot be excluded; verify specificity with target knockout or knockdown controls.
• The immunogen spans the N-terminal 292 amino acids; performance may vary across splice variants; confirm recognition using recombinant proteins or cell lines expressing specific isoforms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering this PHF11 polyclonal antibody alongside a broad portfolio of validated immune-related antibodies and recombinant proteins. We support diagnostic manufacturers and researchers with optimized antibody pairs, bulk reagent supply, and customized sourcing for rare targets like transcription regulators. Contact us to streamline your assay development from concept to clinic.