Anti-PHD3 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9H6Z9

Core Product Specifications and Parameters

Biological Background: PHD3 Function and Localization

• PHD3, also known as Egl nine homolog 3, HIF-prolyl hydroxylase 3 (HIF-PH3), and prolyl hydroxylase domain-containing protein 3 (PHD3), is encoded by the EGLN3 gene and is a prolyl hydroxylase that mediates hydroxylation of proline residues in target proteins such as PKM, TELO2, ATF4, GPX4, and HIF1A (PubMed:19584355, PubMed:20978507, PubMed:21483450). Related references: PMID:19584355, PMID:20978507, PMID:21483450
• As a cellular oxygen sensor, under normoxic conditions PHD3 catalyzes hydroxylation of specific proline residues in hypoxia-inducible factor alpha (HIF1A and HIF2A), targeting them for proteasomal degradation via the von Hippel-Lindau complex, thereby attenuating the hypoxic response (PubMed:11595184, PubMed:12181324). Related references: PMID:11595184, PMID:12181324
• PHD3 acts as an inhibitor of ferroptosis by hydroxylating GPX4, preventing its degradation via chaperone-mediated autophagy; this process is promoted by PSAT1 providing 2-oxoglutarate substrate (PubMed:40281343). Related references: PMID:40281343
• It also hydroxylates PKM in hypoxia, limiting glycolysis (PubMed:21483450, PubMed:21620138). Related references: PMID:21483450, PMID:21620138
• PHD3 is a regulator of apoptosis: in cardiomyocytes, it inhibits the anti-apoptotic effect of BCL2 by disrupting the BAX-BCL2 complex (PubMed:20849813); in neurons, it has a NGF-induced proapoptotic effect by regulating CASP3 activity (PubMed:16098468). Related references: PMID:20849813, PMID:16098468
• The protein is essential for hypoxic regulation of neutrophilic inflammation (PubMed:21317538) and plays a crucial role in DNA damage response by hydroxylating TELO2, promoting its interaction with ATR for activation of the ATR/CHK1/p53 pathway (PubMed:22797300). Related references: PMID:21317538, PMID:22797300
• PHD3 localizes to both the nucleus and cytoplasm and is widely expressed at low levels, with higher expression in adult heart, lung, placenta, and pancreas (acini and islet cells), and in fetal spleen, heart, and skeletal muscle.
• Keywords associated with PHD3 include apoptosis, cytoplasm, dioxygenase, DNA damage, iron, metal-binding, nucleus, oxidoreductase, proteomics identification, reference proteome, Ubl conjugation, and vitamin C.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the N-terminal region (aa 1-100) of human PHD3, which is conserved across human, mouse, and rat (UniProt Q9H6Z9). The resulting polyclonal antibody is expected to recognize both native and denatured PHD3, suitable for multiple applications.
• For Western blotting (WB), a band around 27 kDa is expected. Optimal antibody dilution should be determined experimentally, as batch-to-batch variability is inherent to polyclonal antibodies.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), consider antigen retrieval methods appropriate for formalin-fixed paraffin-embedded sections. Validate staining specificity in tissues with known expression (e.g., adult heart, lung, placenta).
• For ELISA, the antibody may be used as capture or detection reagent depending on the assay format. Screen different coating concentrations and blocking conditions for best signal-to-noise ratio.
• Cross-reactivity with mouse and rat is supported by sequence alignment; nevertheless, validation in the relevant sample system is recommended before large-scale use.
• Due to the polyclonal nature, lot-specific performance may vary. Include appropriate positive and negative controls (e.g., PHD3-knockout cell lysates) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement, providing validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents for diagnostic manufacturers and research laboratories. This anti-PHD3 polyclonal antibody supports hypoxia, apoptosis, ferroptosis, and DNA damage research, complementing CamelBio’s expertise in rare target material sourcing. From concept to clinic, our integrated technical and supply solutions help accelerate your assay development.