Anti-PHD2/EGLN1 Rabbit Monoclonal Antibody for WB, ELISA - Q9GZT9

Core Product Specifications and Parameters

Biological Background: PHD2/EGLN1 Function and Localization

• PHD2/EGLN1, also known as Egl nine homolog 1 or HIF-prolyl hydroxylase 2 (HIF-PH2), is a cellular oxygen sensor belonging to the prolyl hydroxylase domain-containing protein family.
• Under normoxic conditions, it catalyzes the hydroxylation of specific proline residues within the oxygen-dependent degradation domain of hypoxia-inducible factor alpha (HIF-α) subunits, marking them for von Hippel-Lindau-mediated ubiquitination and proteasomal degradation.
• This enzymatic activity requires iron, 2-oxoglutarate, and vitamin C as cofactors, and is attenuated under hypoxia, allowing HIF-α stabilization and translocation to the nucleus to induce hypoxic gene expression.
• The protein localizes to both the cytoplasm and nucleus, and is widely expressed in human tissues, with highest levels in heart, brain, skeletal muscle, and kidney.
• Post-translational modifications include acetylation, phosphorylation, and S-nitrosylation, which may modulate its activity; alternative splicing generates multiple isoforms.
• Mutations in EGLN1 are associated with congenital erythrocytosis type 3 (ECYT3), highlighting its critical role in erythropoiesis and oxygen homeostasis.

Experimental Guidance and Technical Tips

• The antibody is raised against a synthetic peptide spanning amino acids 1-100 of human PHD2/EGLN1. Consider that this N-terminal epitope may detect multiple isoforms or cleavage fragments; validate the observed bands in your specific experimental context.
• For Western blot, the predicted molecular weight is approximately 46 kDa. Using positive controls such as lysates from normoxic versus hypoxic cells can help confirm the specificity of the signal.
• The antibody is suitable for ELISA and WB on human samples. Cross-reactivity with other species has not been tested; if used in non-human models, thorough validation is recommended.
• Optimal dilution and incubation conditions should be determined empirically for each application and sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw materials, providing validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents to streamline diagnostic development. With deep expertise in hypoxia signaling targets like PHD2/EGLN1, we ensure reliable sourcing for your assay manufacturing needs. From concept to clinic, CamelBio accelerates your workflow with tailored, one-stop access to critical and rare raw materials.