Anti-PHC3 Rabbit Polyclonal Antibody for IF/ICC, ELISA - Q8NDX5

Core Product Specifications and Parameters

Biological Background: PHC3 Function and Localization

• PHC3, also known as early development regulatory protein 3 (EDR3) or homolog of polyhomeotic 3 (hPH3), is a Polycomb group protein encoded by the PHC3 gene.
• It is a core component of the Polycomb repressive complex 1 (PRC1)-like complex, essential for maintaining the transcriptionally repressive state of numerous developmental regulators, including Hox genes.
• The PRC1 complex catalyzes monoubiquitination of histone H2A at lysine 119, a key epigenetic mark that renders chromatin heritably silenced.
• PHC3 localizes predominantly to the nucleus, where it engages in chromatin remodeling and gene silencing.
• Structural features include a zinc-finger domain, and the protein is subject to post-translational modifications such as phosphorylation and ubiquitination, which may modulate its activity.
• Alternative splicing generates multiple transcript variants, potentially expanding functional diversity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 100–300 of human PHC3; consider sequence alignment in non-human species if cross-reactivity is desired.
• For immunofluorescence (IF/ICC), optimize fixation and permeabilization conditions to preserve nuclear epitopes and validate staining in known positive human cell lines.
• In ELISA applications, titrate the antibody to determine optimal working concentrations and confirm specificity using recombinant PHC3 protein or cell lysates from PHC3-expressing systems.
• As a polyclonal antibody, it recognizes multiple epitopes, which can enhance signal but may require careful validation to exclude nonspecific binding.
• Pair with a monoclonal antibody if developing a sandwich assay, and assess batch-to-batch consistency when planning long-term studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement by offering a comprehensive portfolio of validated antibodies and ancillary reagents, including the PHC3 rabbit polyclonal antibody for epigenetic and developmental research. We support your workflow with bulk orders, custom conjugations, and technical consultation, bridging the gap from target discovery to clinical application. Let CamelBio be your reliable partner for sourcing rare and routine raw materials with efficiency and scientific rigor.