Anti-PHC3 Polyclonal Antibody for WB, ELISA - Q8NDX5

Core Product Specifications and Parameters

Biological Background: PHC3 Function and Localization

• Polyhomeotic-like protein 3 (PHC3), also known as early development regulatory protein 3 or homolog of polyhomeotic 3 (hPH3), is a component of the Polycomb group (PcG) multiprotein PRC1-like complex.
• The PRC1 complex maintains the transcriptionally repressive state of many developmental genes, including Hox genes, through chromatin remodeling and histone modification.
• PHC3 contributes to PRC1-mediated monoubiquitination of histone H2A at 'Lys-119', a key epigenetic mark that heritably alters chromatin expressibility.
• The protein localizes to the nucleus, consistent with its role in chromatin regulation and gene silencing.
• PHC3 belongs to the polyhomeotic family and contains characteristic zinc-finger domains, indicating DNA-binding and metal-ion coordination properties.
• Post-translational modifications include phosphorylation and ubiquitin-like conjugation (isopeptide bond formation), suggesting dynamic regulation of its activity or stability.
• Alternative splicing of PHC3 generates multiple isoforms, potentially expanding functional diversity in different tissues or developmental stages.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 100-300 of human PHC3 (NP_079223.3), a region likely conserved among the reported cross-reactive species; consider sequence alignment to confirm epitope conservation.
• For Western blot, the expected molecular weight is approximately 106 kDa; ensure adequate protein transfer and membrane blocking for high-molecular-weight detection.
• In ELISA applications, titrate the antibody against the recombinant protein or cell lysates to determine optimal working concentrations.
• When using this polyclonal antibody, validate specificity in relevant knockout or knockdown models if available, as polyclonal preparations may detect multiple isoforms.
• Due to the nuclear localization of PHC3, strong lysis and denaturing conditions may be required for efficient extraction and detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For targets like PHC3 involved in chromatin and Polycomb group protein research, we offer reliable sourcing solutions and technical support to streamline your assay development from concept to clinic.