Anti-PHC2 Polyclonal Antibody for WB and ELISA - Q8IXK0

Core Product Specifications and Parameters

Biological Background: PHC2 Function and Localization

• PHC2 is a core component of the Polycomb repressive complex 1 (PRC1), which maintains transcriptional repression of target genes, such as Hox genes, throughout development.
• The PRC1 complex mediates chromatin remodeling and histone modifications, with PHC2 contributing to the monoubiquitination of histone H2A at Lys-119, leading to heritable gene silencing.
• PHC2 is localized to the nucleus, consistent with its role in chromatin regulation and epigenetic memory.
• It belongs to the polyhomeotic family and contains zinc-finger domains, enabling DNA-binding activity; alternative splicing generates multiple isoforms.
• Post-translational modifications include phosphorylation and ubiquitination, as indicated by proteomics data and keyword annotations.
• Also known as early development regulatory protein 2 (EDR2), PHC2 plays a critical role in early embryonic development and cell fate determination.

Experimental Guidance and Technical Tips

• For Western blot, consider using whole-cell lysates from human cell lines; a band at approximately 91 kDa may be detected. Validate with appropriate positive and negative controls.
• In ELISA, test against the recombinant immunogen (aa 250-323) to confirm antibody reactivity; optimize coating concentrations and detection conditions.
• Since PHC2 functions in the nucleus, nuclear extract preparations may enrich the target for WB analysis.
• The antibody is raised in rabbit, so use anti-rabbit secondary antibodies for detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive sourcing solutions for IVD raw materials, including this PHC2 antibody for chromatin biology and epigenetics research. We offer validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets, supporting diagnostic manufacturers and laboratories from concept to clinic.