Anti-PHC1 Rabbit Polyclonal Antibody for WB, ELISA - P78364

Core Product Specifications and Parameters

Biological Background: Polyhomeotic-like protein 1 Function and Localization

• Polyhomeotic-like protein 1 (PHC1), also designated Early development regulatory protein 1 (EDR1), is encoded by the PHC1 gene (aliases EDR1, PH1).
• PHC1 is a core component of the Polycomb repressive complex 1 (PRC1), which maintains the transcriptionally repressed state of developmental regulators such as Hox genes.
• The PRC1 complex mediates monoubiquitination of histone H2A at Lys-119, leading to chromatin compaction and heritable gene silencing.
• PHC1 is required for proper control of cellular levels of geminin (GMNN) expression, linking it to cell cycle regulation.
• The protein localizes to the nucleus and contains zinc-finger domains, enabling DNA-binding and metal-ion coordination.
• Post-translational modifications include phosphorylation, isopeptide bond formation, and ubiquitin-like conjugation.
• Clinically, variants in PHC1 are associated with primary microcephaly, underscoring its developmental importance.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 750–940 of human PHC1 (C-terminal region); consider epitope accessibility when designing downstream assays.
• For Western blotting, the predicted molecular weight is ~106 kDa; validate band specificity using appropriate positive and negative controls.
• In ELISA applications, ensure the coating antigen matches the immunogen or use a validated protocol; cross-reactivity with mouse and rat suggests conserved epitopes, but independent verification in the relevant model system is advised.
• Store as recommended and minimize freeze–thaw cycles to preserve antibody integrity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw materials, offering a one-stop procurement bridge from concept to clinic. This anti-PHC1 polyclonal antibody complements our portfolio for epigenetics and developmental biology research. We provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare-target raw materials to streamline your diagnostic development.