Anti-PHACTR1 Polyclonal Antibody for WB, ELISA - Q9C0D0

Core Product Specifications and Parameters

Biological Background: PHACTR1 Function and Localization

• Encoded by the PHACTR1 gene (alternative names: RPEL1, KIAA1733), phosphatase and actin regulator 1 is a ~66 kDa protein belonging to the phosphatase and actin regulator family.
• Binds to actin monomers (G-actin) and modulates actin cytoskeleton dynamics, promoting actin stress fiber formation and influencing cell motility and survival.
  Related references:
  PMID:21798305
  PMID:21939755
• Regulates the activity of protein phosphatase 1 catalytic subunit alpha (PPP1CA), acting as a protein phosphatase inhibitor, thereby impacting various cellular processes.
  Related references:
  PMID:21798305
  PMID:21939755
• Plays a role in endothelial cell tubulogenesis and has been implicated in neuronal migration and dendrite arborization, with variants associated with epilepsy.
  Related references:
  PMID:21798305
  PMID:21939755
• Localized to the cytoplasm, nucleus, and synapses, where it likely performs context-dependent functions.
• Subject to post-translational modification; annotated as a phosphoprotein, and contains RPEL repeats for actin binding, with a known 3D structure.
• Detected in umbilical vein endothelial cells, consistent with its role in endothelial function.

Experimental Guidance and Technical Tips

• For Western blot, a band of approximately 66 kDa is expected; include appropriate positive controls such as endothelial cell lysates where PHACTR1 expression is reported.
• Validate ELISA performance using recombinant PHACTR1 or cell lysates from species of interest, optimizing coating and detection conditions.
• Cross-reactivity with mouse and rat suggests a conserved epitope; verify specificity with appropriate species‑matched controls.
• As a polyclonal antibody, batch-to-batch consistency should be confirmed; consider lot-specific validation in critical assays.
• Store aliquots at -20°C upon receipt; avoid repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one‑stop access to a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies like this PHACTR1 antibody, and bulk ancillary reagents. Our integrated supply chain supports your development from concept to clinic, enabling efficient sourcing for actin cytoskeleton research and related diagnostic applications. Whether you need rare‑target raw‑material sourcing or scalable production, we bridge your requirements with reliable supply solutions.