Anti-PGRMC1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O00264

Core Product Specifications and Parameters

Biological Background: PGRMC1 Function and Localization

• Membrane-associated progesterone receptor component 1 (PGRMC1), also known as Dap1 and IZA, is a single-pass membrane protein.
• Functions as a progesterone-binding protein within a progesterone receptor complex (PubMed:28396637) and binds progesterone directly (PubMed:25675345). Related references: PMID:28396637 PMID:25675345
• Acts as an intracellular heme chaperone, regulating heme synthesis through interaction with FECH and serving as a heme donor to certain hemoproteins (PubMed:27599036). Related references: PMID:27599036
• Forms a ternary complex with TMEM97 and LDLR, enhancing LDLR-mediated LDL internalization (PubMed:30443021). Related references: PMID:30443021
• Localizes to smooth endoplasmic reticulum and mitochondrial outer membranes, and is also secreted.
• Widely expressed, with highest levels in liver and kidney; detected in urine and endometrial tissue (PubMed:36213313, PubMed:37453717, PubMed:23793472). Related references: PMID:36213313 PMID:37453717 PMID:23793472
• Undergoes post-translational modifications including phosphorylation and glycosylation, and possesses a transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen (aa 44-195) targets the N-terminal region of PGRMC1 and may detect both full-length and alternatively spliced isoforms.
• Suitable for Western blot analysis with a predicted band around 22 kDa; optimize loading and transfer conditions for consistent detection.
• For IHC-P and IF/ICC, consider antigen retrieval and permeabilization steps tailored to your tissue or cell type.
• Perform initial titration in your experimental system to determine optimal dilution for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, supporting progesterone receptor and heme homeostasis research with high-quality antibodies like this anti-PGRMC1 polyclonal antibody. We supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and facilitate rare target raw-material procurement—streamlining your transition from discovery to diagnostic application.