Anti-PGP9.5/UCHL1 Rabbit Monoclonal Antibody for WB, IF/ICC, IF-F, IF-P, IHC-P, ELISA - P09936

Core Product Specifications and Parameters

Biological Background: PGP9.5/UCHL1 Function and Localization

• Recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin, maintaining a stable pool of monoubiquitin essential for the ubiquitin-proteasome and autophagy-lysosome pathways. Related references: PMID:12408865 | PMID:8639624 | PMID:9774100
• Plays a role in the regulation of synaptic function, cardiac function, inflammatory response, and osteoclastogenesis. Related references: PMID:22212137 | PMID:23359680
• Abrogates the ubiquitination of multiple proteins including WWTR1/TAZ, EGFR, HIF1A, and BACE1; regulates APP processing by promoting BACE1 degradation, reducing amyloid-beta production. Related references: PMID:22212137 | PMID:25615526
• Enhances HIF1A activity by preventing its VHL E3 ligase-mediated ubiquitination and subsequent degradation. Related references: PMID:25615526
• Located in the cytoplasm and endoplasmic reticulum membrane.
• Expressed primarily in neurons and neuroendocrine cells; down-regulated in brains of Parkinson's and Alzheimer's disease patients.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human UCHL1 (aa 1–220); consider using full-length recombinant protein or lysates for positive controls.
• For Western blot, a band near 25 kDa is expected; validate with appropriate molecular weight markers and consider using reducing conditions.
• For immunofluorescence (IF/ICC, IF-F, IF-P), optimize fixation and permeabilization as protein localizes to cytoplasm and ER membrane.
• For immunohistochemistry (IHC-P), antigen retrieval may improve detection; include neuronal tissue controls.
• The antibody shows cross-reactivity with mouse, rat, and monkey; verify in the relevant sample system if using non-human species.
• In ELISA applications, pair with a suitable detection antibody and optimize coating conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement for diagnostic manufacturers and researchers, offering validated antibody pairs, bulk ancillary reagents, and rare target sourcing. This PGP9.5/UCHL1 monoclonal antibody supports neurodegeneration and ubiquitin pathway studies, and we can assist with complementary reagents or customized logistics to accelerate your project from concept to clinic.