Anti-PGP9.5/UCHL1 Polyclonal Antibody for WB, IF/ICC, ELISA - P09936

Core Product Specifications and Parameters

Biological Background: PGP9.5/UCHL1 Function and Localization

• PGP9.5/UCHL1 encodes ubiquitin carboxyl-terminal hydrolase isozyme L1, a deubiquitinase involved in maintaining the monoubiquitin pool by hydrolyzing the C-terminal glycine of ubiquitin, essential for ubiquitin-proteasome and autophagy-lysosome pathways. Related references: PMID:12408865 PMID:8639624 PMID:9774100
• It regulates diverse processes—synaptic function, cardiac function, inflammatory response, and osteoclastogenesis—by deubiquitinating specific target proteins. Related references: PMID:22212137 PMID:23359680
• In Alzheimer’s disease-relevant pathways, UCHL1 modulates amyloid precursor protein processing by promoting BACE1 degradation, leading to decreased amyloid beta production. Related references: PMID:22212137
• The protein stabilizes hypoxia-inducible factor 1-alpha (HIF1A) by abrogating its VHL-mediated ubiquitination and degradation, thereby enhancing HIF1A transcriptional activity. Related references: PMID:25615526
• Subcellularly, UCHL1 is primarily cytoplasmic and localizes to the endoplasmic reticulum membrane.
• It exhibits strong tissue specificity, being highly expressed in neurons and diffuse neuroendocrine system cells; expression is significantly down-regulated in the brains of Parkinson’s and Alzheimer’s disease patients.
• Post-translational modifications include acetylation, phosphorylation, prenylation, and oxidation; the protein is also a lipoprotein and glycoprotein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human UCHL1 (residues 1–220). For western blotting (WB), anticipate a band near the theoretical molecular weight of ~25 kDa, but verify with appropriate positive controls (e.g., human, mouse, or rat neural tissues).
• In immunofluorescence/immunocytochemistry (IF/ICC), optimize fixation and permeabilization conditions to preserve antigenicity and access cytoplasmic epitopes.
• For ELISA applications, the polyclonal nature of this antibody may provide sensitive detection of UCHL1; consider using a matched monoclonal pair for sandwich ELISA if quantitative accuracy is required.
• Cross-reactivity with mouse and rat is indicated; however, sequence homology should be checked for other species, and validation in the intended experimental system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. This anti-PGP9.5/UCHL1 polyclonal antibody supports neuroscience and neurodegenerative disease research, from early biomarker discovery to assay development. For rare target sourcing or customized production, CamelBio’s integrated supply chain and technical expertise help accelerate your project from concept to clinic.