Anti-PGM1 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P36871

Core Product Specifications and Parameters

Biological Background: PGM1 Function and Localization

• PGM1 (Phosphoglucomutase-1), also known as Glucose phosphomutase 1, CDG1T, and GSD14, is a key enzyme in carbohydrate metabolism.
• It catalyzes the reversible isomerization of alpha-D-glucose 1-phosphate to alpha-D-glucose 6-phosphate via a glucose-1,6-bisphosphate intermediate, linking glycogen breakdown and glucose synthesis. Related references: PMID:15378030, PMID:25288802, PMID:17924679
• PGM1 belongs to the phosphoglucomutase family and is essential for maintaining cellular glucose homeostasis.
• Subcellularly, PGM1 is predominantly localized in the cytoplasm.
• Post-translational modifications include acetylation and phosphorylation, and the protein is metal-dependent (magnesium).
• Mutations in PGM1 cause congenital disorder of glycosylation type 1T (CDG1T) and glycogen storage disease type XIV (GSD14).
• The calculated molecular weight is approximately 61 kDa, and the protein has been identified through proteomics.

Experimental Guidance and Technical Tips

• For Western blot (WB), a band around 61 kDa is expected; validate in your specific sample system due to possible post-translational modifications or isoforms.
• The immunogen corresponds to amino acids 465-562 of human PGM1; consider sequence alignment with homologous proteins if cross-reactivity is a concern.
• In immunofluorescence (IF/ICC), permeabilization is required as the target is cytoplasmic; use appropriate controls to confirm specificity.
• ELISA application may require optimization of coating and detection conditions; the antibody is suitable for sandwich or direct formats.
• When using across species (human, mouse, rat), verify sequence homology in the immunogen region to ensure reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic developers and researchers with integrated access to premium IVD raw materials, including validated monoclonal antibodies like this anti-PGM1 clone. Whether you need optimized antibody pairs, bulk reagents, or sourcing for rare targets, our supply chain supports the full spectrum of glucose metabolism and clinical enzyme research. Let CamelBio bridge your project from concept to clinic with reliable, one-stop procurement.