Anti-PGLYRP2 Polyclonal Antibody for WB, ELISA - Q96PD5

Core Product Specifications and Parameters

Biological Background: PGLYRP2 Function and Localization

• PGLYRP2, also known as peptidoglycan recognition protein 2 or PGRP-L, is a member of the peptidoglycan recognition protein family, which plays a crucial role in innate immunity.
• It functions as an N-acetylmuramoyl-L-alanine amidase, hydrolyzing the amide bond between N-acetylmuramic acid and L-alanine residues in bacterial peptidoglycan.
• The protein acts as a scavenger by digesting biologically active peptidoglycan (PGN) into inactive fragments, thereby modulating immune responses, but has no direct bacteriolytic activity.
• Alternative splicing generates multiple isoforms; isoform 2 is not detected in the liver or serum, suggesting tissue-specific regulation.
• PGLYRP2 is strongly expressed in the liver and fetal liver, and is secreted into the serum; lower expression is observed in transverse colon, lymph nodes, heart, thymus, pancreas, descending colon, stomach, and testis.
• Subcellularly, the protein is secreted and also found associated with membranes, consistent with its role in extracellular peptidoglycan processing.
• The protein contains key structural features including a signal peptide, a zinc-binding domain essential for its amidase activity, disulfide bonds, and glycosylation sites; it is also regulated by phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from amino acids 300–400 of human PGLYRP2; therefore, the antibody may detect multiple isoforms containing this region.
• For Western blot (WB), the predicted molecular weight is ~62 kDa, but due to glycosylation and phosphorylation, the observed band may appear at a higher or different molecular weight; validate with appropriate controls.
• This antibody cross-reacts with mouse PGLYRP2, making it suitable for studies in mouse models; however, validate specificity in the relevant tissue or cell lysate.
• For ELISA applications, consider using the immunizing peptide as a positive control and titrate the antibody to determine optimal working dilutions.
• The recommended storage and shipping condition is on ice bags; avoid repeated freeze-thaw cycles to maintain activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio stands as your comprehensive partner for IVD raw materials, offering everything from concept to clinic. Our catalog includes high-quality polyclonal and monoclonal antibodies, such as this PGLYRP2 antibody, essential for innate immunity and peptidoglycan research. Whether you're scaling up production or developing new diagnostic assays, CamelBio provides validated antibody pairs, bulk reagents, and customized sourcing solutions to meet your needs.