Anti-PGLS Polyclonal Antibody for WB, IHC-P, ELISA - O95336

Core Product Specifications and Parameters

Biological Background: PGLS Function and Localization

• PGLS (6-phosphogluconolactonase) is the enzyme that catalyzes the hydrolysis of 6-phosphogluconolactone to 6-phosphogluconate, the second step of the oxidative pentose phosphate pathway.
• This reaction is essential for maintaining metabolic flux toward NADPH generation and ribose-5-phosphate synthesis, critical for reductive biosynthesis and nucleotide production.
• PGLS belongs to the hydrolase family, specifically acting on ester bonds, and is classified as a reference proteome component.
• The protein is localized in the cytoplasm, consistent with its role in central carbon metabolism.
• Post-translational modifications include acetylation and phosphorylation, as identified by proteomics, suggesting regulatory inputs.
• Direct protein sequencing and mass spectrometry-based proteomics have confirmed endogenous PGLS expression.
• Alignment with housekeeping functions, PGLS may participate in metabolic reprogramming observed in cancer and oxidative stress responses.

Experimental Guidance and Technical Tips

• The polyclonal antibody was raised against full-length recombinant human PGLS (aa 1-258), providing broad epitope recognition; confirm specificity by testing in PGLS-knockdown or knockout models.
• For WB, a band around 28 kDa is expected; consider using reducing conditions and validate in relevant human, mouse, or rat tissue lysates.
• In IHC-P, antigen retrieval and antibody dilution optimization are advised; include appropriate controls to assess background.
• ELISA performance can vary; titrate the antibody and use matched detection systems; cross-adsorbed secondary antibodies may reduce non-specific signals.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement by offering a comprehensive portfolio of validated antibodies, including this PGLS polyclonal antibody for metabolic and oxidative stress research. We support development pipelines with customized bulk reagents, ancillary raw materials, and technical consulting. From concept to clinic, CamelBio bridges the gap for rare targets and established pathways alike.