Anti-PGK1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P00558

Core Product Specifications and Parameters

Biological Background: PGK1 Function and Localization

• PGK1 (Phosphoglycerate kinase 1) is encoded by the PGK1 gene (GeneID: 5230) and also known as PGKA, MIG10, and HEL-S-68p. The protein has alternative names including Cell migration-inducing gene 10 protein and Primer recognition protein 2 (PRP 2).
• It catalyzes a key ATP-producing step in glycolysis: the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. Related references: PMID:30323285 PMID:7391028
• PGK1 shows broad nucleotide substrate specificity, utilizing both L- and D- forms of purines and pyrimidines, though activity on pyrimidines is lower. Related references: PMID:18463139
• Beyond its metabolic role, PGK1 acts as a polymerase alpha cofactor (primer recognition protein). Related references: PMID:2324090
• In the mitochondrion, PGK1 functions as a protein kinase, phosphorylating PDK1 to inhibit the pyruvate dehydrogenase complex, thereby suppressing oxidative phosphorylation and promoting glycolysis. Related references: PMID:26942675 PMID:36849569
• PGK1 may contribute to sperm motility regulation. Related references: PMID:26677959
• Subcellularly, PGK1 localizes to the cytoplasm, cytosol, and mitochondrial matrix.
• Tissue-specific expression is prominent in spermatogonia and sperm principle piece, with reduced levels in aged testis.
• Post-translational modifications include acetylation and phosphorylation, as indicated by keyword analysis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to nearly the full-length human PGK1 protein, which may yield broad epitope recognition; validate in your specific sample system.
• For Western blotting (WB), expect a band around 45 kDa; consider using appropriate loading controls and validate in tissues with known PGK1 expression.
• For immunohistochemistry (IHC-P), test the antibody on tissues with expected PGK1 positivity (e.g., testis, high-glycolytic tumors) and include negative controls.
• For ELISA, the antibody can be used as a detection reagent; optimize coating and blocking conditions based on your experimental design.
• The antibody cross-reacts with mouse and rat PGK1; verify detection sensitivity and specificity in these species through pilot experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and custom rare-target procurement. This anti-PGK1 antibody supports research in glycolysis, mitochondrial biology, and reproductive studies, and our network can help streamline your assay development from concept to clinic.