Anti-PGHS-2 Polyclonal Antibody for WB - P35354

Core Product Specifications and Parameters

Biological Background: PGHS-2 (COX-2/PTGS2) Function and Localization

• Prostaglandin G/H synthase 2 (PGHS-2), also known as cyclooxygenase-2 (COX-2), is a dual-function enzyme that catalyzes the conversion of arachidonic acid to prostaglandin H2 (PGH2) via sequential cyclooxygenation and peroxidation reactions.
  Related references:
  PMID:16373578, PMID:26859324
• It plays a crucial role in the inflammatory response by producing pro-inflammatory prostanoids, and its expression is rapidly induced by cytokines, growth factors, and mitogens.
  Related references:
  PMID:11939906, PMID:16373578, PMID:19540099
• Beyond arachidonic acid, PGHS-2 metabolizes other polyunsaturated fatty acids such as dihomo-γ-linolenate and eicosapentaenoate to generate alternative series of prostaglandins and specialized pro-resolving mediators (resolvins) from docosahexaenoate and eicosapentaenoate.
  Related references:
  PMID:11939906, PMID:19540099, PMID:12391014
• Subcellular localization includes the endoplasmic reticulum membrane, microsomes, and both inner and outer nuclear membranes, facilitating compartment-specific prostanoid synthesis.
• The protein is post-translationally modified by N-glycosylation, acetylation, and S-nitrosylation, and its catalytic activity relies on a heme-containing active site with bound iron.
• PGHS-2 is a member of the prostaglandin G/H synthase family and is essential for lipid mediator biosynthesis in various tissues.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide spanning amino acids 505–604 of human PGHS-2, a region near the C-terminus. This design may confer high specificity for the human protein; however, cross-reactivity with orthologs from other species should be validated experimentally if required.
• For Western blot analysis, PGHS-2 typically migrates at approximately 69–72 kDa under reducing conditions. We recommend using positive control lysates from cells known to express COX-2 (e.g., LPS-stimulated human monocytes or colorectal carcinoma cell lines) to confirm band identity.
• As a polyclonal antibody, it recognizes multiple epitopes on the target protein, offering high sensitivity. Nevertheless, optimization of blocking reagents, antibody dilution, and incubation conditions is essential for minimal background and the best signal-to-noise ratio.
• Upon receipt, store the antibody at -20°C; avoid repeated freeze-thaw cycles to preserve binding activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for premium IVD raw materials, supporting diagnostic manufacturers and research laboratories from early development through clinical validation. Our portfolio includes validated antibodies, such as this anti-PGHS-2 (COX-2) polyclonal antibody for inflammation and prostanoid research, along with bulk ancillary reagents and rare-target material sourcing. Partner with CamelBio to streamline your supply chain and accelerate your diagnostic innovations.