Anti-PGE receptor EP3 (PTGER3) Rabbit Polyclonal Antibody for WB, ELISA - P43115

Core Product Specifications and Parameters

Biological Background: PTGER3 Function and Localization

• PTGER3 encodes the Prostaglandin E2 receptor EP3 subtype (PGE2-R, prostanoid EP3 receptor), a G protein-coupled receptor (GPCR) belonging to the rhodopsin-like receptor family. Related references: PMID:7883006 PMID:7981210 PMID:8117308
• Alternative splicing generates multiple isoforms (EP3, EP3e, EP3-I, EP3-II, EP3-IV, EP3-VI, EP3-III) that may differ in C-terminal tails and signaling properties.
• The receptor couples to G(i) proteins to inhibit adenylate cyclase and can also elevate intracellular calcium, mediating diverse physiological responses to PGE2.
• PTGER3 plays essential roles in fever development (response to pyrogens like IL1B and LPS), platelet aggregation potentiation, and duodenal mucosal protection via increased bicarbonate secretion.
• Subcellular localization is primarily at the cell membrane, consistent with its function as a transmembrane receptor.
• Tissue expression includes kidney, small intestine, heart, pancreas, gastric fundic mucosa, mammary artery, and pulmonary vessels. Related references: PMID:8117308 PMID:8135729
• As a membrane protein, PTGER3 is subject to post-translational modifications including glycosylation and palmitoylation, which may regulate receptor stability and signaling.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-53) of human PTGER3, which is conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blotting, the predicted molecular weight is 43kDa; optimize gel percentage and transfer conditions accordingly.
• Validate antibody specificity in positive control tissues such as kidney or small intestine, where PTGER3 expression is well-documented.
• This polyclonal antibody may detect multiple isoforms due to alternative splicing; confirm band patterns using appropriate controls.
• In ELISA applications, consider using recombinant protein as standard to establish assay sensitivity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we provide diagnostic manufacturers and research labs with a one-stop procurement solution for IVD raw materials, from concept to clinic. This anti-PTGER3 antibody supports studies in prostaglandin signaling, inflammation, and platelet function, and we can further source validated antibody pairs, bulk ancillary reagents, and rare-target raw materials to accelerate your assay development. Whether you need optimized polyclonal antibodies or customized sourcing, CamelBio bridges the gap between discovery and scalable supply.