Anti-PGE receptor EP1 (PTGER1) Rabbit Polyclonal Antibody for WB, ELISA - P34995

Core Product Specifications and Parameters

Biological Background: PGE receptor EP1 (PTGER1) Function and Localization

• Prostaglandin E2 receptor EP1 subtype (PTGER1, also known as prostanoid EP1 receptor) is a G protein-coupled receptor that specifically binds prostaglandin E2 (PGE2).
• PTGER1 is coupled to G(q) proteins and activates a phosphatidylinositol-calcium second messenger system upon ligand binding.
• This receptor modulates renal function and is implicated in the smooth muscle contractile response to PGE2 in various tissues.
• PTGER1 is localized to the cell membrane, consistent with its role as a transmembrane receptor.
• Tissue expression is highest in the kidney, with lower levels in lung, skeletal muscle, and spleen; expression is lowest in testis and undetectable in liver, brain, and heart.
• Post-translational modifications include disulfide bonds and glycosylation; the receptor is also subject to phosphorylation.
• Structural studies have determined the 3D structure of PTGER1.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide mapping to the C-terminal intracellular region (amino acids 300-400) of human PTGER1, suggesting the antibody may recognize the full-length receptor in denaturing Western blot conditions.
• Given its validation in WB and ELISA, consider optimizing dilution factors and detection conditions in the relevant sample system.
• Cross-reactivity with mouse PTGER1 has been reported; validate species-specific reactivity in your experimental setup if working with mouse tissues or cell lines.
• PTGER1 is a membrane protein; ensure appropriate membrane protein extraction protocols for optimal detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, including this anti-PTGER1 polyclonal antibody for renal function and GPCR research. We supply validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and can assist in rare target sourcing to streamline your diagnostic development from concept to clinic.