Anti-PGD Rabbit Monoclonal Antibody for WB, IF/ICC, IP, ELISA - P52209

Core Product Specifications and Parameters

Biological Background: PGD Function and Localization

• PGD (6-phosphogluconate dehydrogenase, decarboxylating) is also known as 6PGD; it is a key enzyme in the pentose phosphate pathway.
• The enzyme catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO₂, coupled with the reduction of NADP to NADPH, providing reducing power and ribose for biosynthesis.
• PGD belongs to the aldo-keto reductase superfamily and functions as a homodimer; alternative splicing generates multiple isoforms.
• It is predominantly localized in the cytoplasm, where it operates in the glucose-6-phosphate dehydrogenase shunt.
• Post-translational modifications include acetylation and phosphorylation, as indicated by proteomics data.
• The protein is a reference proteome member and plays a role in gluconate utilization and maintenance of cellular redox balance.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the C-terminal region (aa 384-483), ensuring specific recognition of human PGD.
• For Western blotting, the predicted molecular weight is ~53 kDa; validate band specificity using appropriate positive and negative controls.
• In immunofluorescence, permeabilization may be required to access cytoplasmic PGD; consider co-staining with compartment markers.
• For immunoprecipitation, use a standard IP buffer; the antibody's rabbit monoclonal nature offers high affinity and reproducibility.
• ELISA protocols may leverage the antibody for antigen capture or detection; cross-reactivity with mouse and rat allows multi-species studies.

CamelBio: Your One-Stop Sourcing Bridge

As your comprehensive IVD raw-material partner, CamelBio supplies this anti-PGD rabbit monoclonal antibody along with validated antibody pairs, bulk ancillary reagents, and optimization support for diagnostic development. Whether you need rare target sourcing or routine reagent supply, our platform streamlines your research and manufacturing from concept to clinic, especially for metabolic disease and oncology studies.