Anti-PGC1α Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9UBK2

Core Product Specifications and Parameters

Biological Background: PGC1α Function and Localization

• PGC1α is a transcriptional coactivator for steroid receptors and nuclear receptors, significantly enhancing the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter.
  Related references: PMID:10713165, PMID:20005308, PMID:21376232
• Plays an essential role in metabolic reprogramming in response to dietary availability by coordinating the expression of genes involved in glucose and fatty acid metabolism.
  Related references: PMID:10713165, PMID:20005308, PMID:21376232
• Acts as a central regulator of gluconeogenesis, stimulating hepatic gluconeogenesis via increased expression of gluconeogenic enzymes and cooperating with FOXO1 to promote the fasting gluconeogenic program.
  Related references: PMID:16753578, PMID:23142079
• Induces the expression of PERM1 in skeletal muscle in an ESRRA-dependent manner.
  Related references: PMID:23836911
• Involved in integrating circadian rhythms and energy metabolism, required for oscillatory expression of clock genes (e.g., BMAL1, NR1D1) and metabolic genes (e.g., PDK4, PEPCK) through coactivation of RORA and RORC.
• Subcellular localization includes the nucleus, PML bodies, and cytoplasm.
• Tissue specificity: Highly expressed in heart, skeletal muscle, liver, and kidney; lower levels in brain and pancreas; very low in intestine and white adipose tissue. In skeletal muscle, levels are lower in obese subjects, and fasting induces a 2-fold increase.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 610–798 of human PGC1α, a region that may be conserved across species; verify sequence alignment if using non-human models.
• Suitable for WB, IHC-P, IF/ICC, and ELISA; for IHC-P and IF/ICC, optimize fixation and antigen retrieval for your tissue or cell type.
• Predicted molecular weight of 91 kDa; include appropriate molecular weight markers and consider post-translational modifications that may alter migration.
• Cross-reacts with mouse and rat, making it potentially useful for rodent metabolic studies; however, confirm reactivity in your specific model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing, supporting every stage from concept to clinic. This anti-PGC1α polyclonal antibody addresses research into metabolic regulation, transcriptional coactivation, and circadian biology. Beyond antibodies, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your diagnostic development.