Anti-PGC1 alpha Rabbit Polyclonal Antibody - O70343

Core Product Specifications and Parameters

Biological Background: PGC1 alpha Function and Localization

• PGC1 alpha (peroxisome proliferator-activated receptor gamma coactivator 1-alpha) is a transcriptional coactivator that interacts with nuclear receptors, notably PPARG and thyroid hormone receptor, to enhance the transcription of genes involved in energy metabolism. Related references: PMID:12754525 PMID:15744310 PMID:23217713
• Functions as a master regulator of mitochondrial biogenesis and oxidative metabolism, and plays an essential role in adaptive thermogenesis. Related references: PMID:12754525 PMID:15744310 PMID:23217713
• Orchestrates metabolic reprogramming in response to dietary availability by coordinating genes involved in glucose and fatty acid metabolism. Related references: PMID:12754525 PMID:15744310 PMID:23217713
• Acts as a key regulator of gluconeogenesis in the liver, stimulating the expression of gluconeogenic enzymes in cooperation with FOXO1 to promote the fasting response. Related references: PMID:12754525
• Participates in the integration of circadian rhythms with energy metabolism through coactivation of RORA and RORC, and is required for oscillatory expression of clock and metabolic genes. Related references: PMID:17476214
• Predominantly localizes to the nucleus, particularly within PML bodies, where it exerts its coactivator functions.
• Highly expressed in tissues with high mitochondrial content, including skeletal muscle, brown adipose tissue, heart, kidney, and brain.
• Subject to post-translational modifications such as acetylation, phosphorylation, and ubiquitination, which modulate its activity and stability.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 1–300 of mouse PGC1 alpha, a region near the N-terminus; this design may recognize multiple isoforms or cleavage products—validate specificity in your experimental system.
• For Western blotting, expect a main band around 91 kDa in lysates from PGC1 alpha-rich tissues (skeletal muscle, brown adipose tissue, heart); additional bands may occur.
• In immunofluorescence and immunohistochemistry, nuclear staining is anticipated; optimize fixation and permeabilization conditions, and include appropriate controls.
• The antibody is suitable for ELISA; titrate to determine the optimal working concentration for your assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, bulk ancillary reagents, and custom procurement of rare targets. For metabolic and transcriptional research, we supply high-quality PGC1 alpha antibodies and related reagents to support diagnostic development and basic studies. Contact our team for reliable, one-stop access to the materials you need from concept to clinic.