Anti-PGBD1 Rabbit Polyclonal Antibody for WB and ELISA - Q96JS3

Core Product Specifications and Parameters

Biological Background: PGBD1 Function and Localization

• PGBD1, also known as SCAND4 or Cerebral protein 4, is a domesticated transposase derived from PiggyBac DNA transposons that has lost transposase activity.
• It has acquired DNA-binding capability and preferentially binds in and around genes involved in neuronal development, inducing transcriptional pausing (PubMed:12955498, PubMed:35609796, PubMed:36205081). Related references: PMID:12955498, PMID:35609796, PMID:36205081
• PGBD1 suppresses paraspeckle assembly in neuronal cells, highlighting its role in nuclear body regulation (PubMed:36205081). Related references: PMID:36205081
• The protein localizes to the nucleus.
• Post-translational modifications include phosphorylation, isopeptide bond formation, and ubiquitin-like conjugation.
• PGBD1 is implicated in neuronal development and function, consistent with its cerebral expression patterns.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an internal region (amino acids 150-450) of human PGBD1, which may enhance target specificity in WB and ELISA applications.
• For WB, the predicted molecular weight is approximately 93 kDa; validate using appropriate molecular weight markers and human neuronal cell line lysates.
• Consider using a nuclear extract as a positive control, given PGBD1's nuclear localization.
• Optimize antibody concentration and detection conditions for each application; non-specific binding should be assessed using relevant negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials for neuroscience and gene regulation research, including validated antibodies against targets like PGBD1. Our supply chain supports bulk procurement of polyclonal and monoclonal antibodies, ancillary reagents, and customized rare-target sourcing. Partner with CamelBio to streamline your diagnostic development pipeline from concept to clinic.