Anti-PGAM5 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q96HS1

Core Product Specifications and Parameters

Biological Background: PGAM5 Function and Localization

• PGAM5 is a mitochondrial serine/threonine protein phosphatase involved in regulating mitochondrial dynamics, mitophagy, oxidative stress response, and necroptosis.
• It dephosphorylates DNM1L/DRP1 to promote mitochondrial fission (PMID:32439975) and MFN2 to promote fusion (PMID:37498743). Related references: PMID:32439975 PMID:37498743
• PGAM5 regulates mitophagy by dephosphorylating FUNDC1 at Ser-13, which enables interaction with LC3 (PMID:24746696). Related references: PMID:24746696
• It forms a tertiary complex with KEAP1 and NRF2 to modulate the anti-oxidative response (PMID:18387606). Related references: PMID:18387606
• In necroptosis, PGAM5 is phosphorylated by RIPK3, leading to recruitment of the RIPK1-RIPK3-MLKL necrosome to mitochondria (PMID:22265414). Related references: PMID:22265414
• PGAM5 localizes to the mitochondrial outer and inner membranes.
• Alternative names include Bcl-XL-binding protein v68 and Phosphoglycerate mutase family member 5; the protein undergoes acetylation, phosphorylation, and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 50–146 of human PGAM5, a region conserved across reported isoforms. For western blot, expect bands around 32 kDa and 28 kDa, consistent with the two major isoforms.
• In immunofluorescence and immunohistochemistry, PGAM5 displays mitochondrial localization; colocalization with mitochondrial markers can confirm specificity.
• The antibody has been validated for ELISA and may be applicable for immunoprecipitation, but users should optimize conditions for their specific assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop partner for IVD raw material supply, from monoclonal antibodies to bulk reagents and technical services. This PGAM5 antibody supports investigations into mitochondrial biology, cell death, and related biomarker discovery. CamelBio can assist with sourcing matched antibody pairs, custom conjugations, and scalable production for your diagnostic kit development needs.