Anti-PGAM5 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q96HS1

Core Product Specifications and Parameters

Biological Background: PGAM5 Function and Localization

• PGAM5 is a serine/threonine-protein phosphatase primarily located in mitochondria, existing as multiple isoforms from alternative splicing. It is also known as BXLBV68 and belongs to the phosphoglycerate mutase family.
• It resides on both the outer and inner mitochondrial membranes, a unique dual topology critical for its diverse functions.
• PGAM5 regulates mitochondrial fission by dephosphorylating DNM1L/DRP1, thereby modulating cellular senescence. Related references: PMID:32439975
• It dephosphorylates MFN2, preventing its ubiquitination and degradation to maintain mitochondrial network integrity. Related references: PMID:37498743
• PGAM5 promotes PARKIN-independent mitophagy by interacting with and dephosphorylating FUNDC1, facilitating its association with LC3. Related references: PMID:24746696
• It participates in the anti-oxidative response by forming a complex with KEAP1 and NRF2. Related references: PMID:18387606
• PGAM5 is a key mediator of necroptosis, acting as a substrate for RIPK3 and recruiting the RIPK1-RIPK3-MLKL necrosome to mitochondria. Related references: PMID:22265414

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within the human PGAM5 protein; verify sequence similarity with target species to ensure expected cross-reactivity.
• For Western blotting, the predicted protein weight is 32 kDa, but actual band position may vary due to isoforms or modifications; validate with appropriate controls.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), mitochondrial localization may require optimized permeabilization and fixation protocols.
• In ELISA applications, titrate antibody and antigen concentrations to achieve optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers efficient sourcing of high-quality IVD antibodies, including this PGAM5 polyclonal antibody, for research in mitochondrial biology, cell death, and related pathways. As a one-stop partner, we supply validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and support for rare target material procurement to accelerate diagnostic development.