Anti-PGAM2 Rabbit Polyclonal Antibody for WB, IF-P, ELISA - P15259

Core Product Specifications and Parameters

Biological Background: PGAM2 Function and Localization

• PGAM2 (Phosphoglycerate mutase 2), also designated muscle-specific phosphoglycerate mutase (PGAM-M), is encoded by the PGAM2 gene.
• The enzyme catalyzes the interconversion of 3-phosphoglycerate and 2-phosphoglycerate in glycolysis, requiring 2,3-bisphosphoglycerate as a primer.
• It can also catalyze the isomerization of (2R)-2,3-bisphosphoglycerate to (2R)-3-phospho-glyceroyl phosphate, though with reduced activity.
• PGAM2 is highly expressed in heart and skeletal muscle, with no detectable expression in liver or brain.
• Mutations in PGAM2 cause glycogen storage disease type X (GSD10), characterized by exercise-induced muscle cramps and myoglobinuria.
• The protein is annotated as a phosphoprotein, indicating potential regulation by phosphorylation.
• Keywords associated with PGAM2 include Disease variant, Glycogen storage disease, Glycolysis, Hydrolase, and Isomerase, reflecting its diverse functional roles.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein encompassing the full-length PGAM2 sequence (amino acids 1-253), which may provide robust detection of the native protein.
• For Western blot, the predicted molecular weight is approximately 29 kDa; validate with positive control lysates from heart or skeletal muscle.
• In immunofluorescence (IF-P), consider using frozen muscle or cardiac tissue sections to evaluate localization; permeabilization and blocking conditions should be optimized.
• For ELISA, this polyclonal antibody can be used as a capture or detection reagent after appropriate titration and pairing with a matched antibody.
• Cross-reactivity with mouse and rat PGAM2 has been reported, but it is advisable to confirm specificity in the relevant species using appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing bridge for IVD raw materials, supporting diagnostic manufacturers and research laboratories from concept to clinic. The PGAM2 polyclonal antibody targets a critical glycolytic enzyme relevant to muscle metabolism and glycogen storage disorders, making it suitable for research into metabolic diseases. CamelBio's supply capabilities include validated antibody pairs, optimized monoclonal and polyclonal antibodies, ancillary reagents, and specialized sourcing for rare target raw materials.