Anti-PGAM1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P18669

Core Product Specifications and Parameters

Biological Background: PGAM1 Function and Localization

• Phosphoglycerate mutase 1 (PGAM1) is a BPG-dependent enzyme that catalyzes the reversible conversion of 2-phosphoglycerate to 3-phosphoglycerate in glycolysis, a critical energy-producing pathway. Related references: PMID:23653202
• PGAM1 also interconverts (2R)-2,3-bisphosphoglycerate and (2R)-3-phospho-glyceroyl phosphate, highlighting its dual isomerase and mutase activities.
• The protein is encoded by the PGAM1 gene (HGNC:8888) and is also known as PGAM-B, PGAMA, and HEL-S-35.
• PGAM1 is predominantly expressed in liver and brain tissues, with little to no expression in muscle, distinguishing it from the muscle-specific PGAM2 isozyme.
• Post-translational modifications include acetylation and phosphorylation, as indicated by proteomics studies, which may regulate its enzymatic activity and cellular localization.
• As a key glycolytic enzyme, PGAM1 is implicated in metabolic reprogramming in cancer and is a potential therapeutic target.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1-254 of human PGAM1, covering the full-length protein. This design may yield antibodies recognizing multiple epitopes.
• For Western blot (WB), expect a predominant band at ~29 kDa. Validate detection in liver or brain lysates, where PGAM1 expression is high.
• For immunofluorescence (IF/ICC), consider using known positive cell lines (e.g., HeLa, HEK293) and include negative controls to confirm specificity.
• ELISA applications may require optimization of antibody concentration and antigen coating conditions; pairing with a validated detection antibody is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, including validated antibodies, antigen-grade proteins, and bulk ancillary reagents. For PGAM1-related research in glycolysis and metabolic disorders, we offer tailored monoclonal and polyclonal antibody solutions to support your assay development. Our integrated supply network accelerates procurement from early-stage R&D to clinical applications.