Anti-PGAM1 Monoclonal Antibody for WB, IHC-P, ELISA - P18669

Core Product Specifications and Parameters

Biological Background: PGAM1 Function and Localization

• Belongs to the phosphoglycerate mutase family and is also known as BPG-dependent PGAM 1 (PGAM-B) or HEL-S-35.
• Central glycolytic enzyme that catalyzes the reversible interconversion of 3-phosphoglycerate and 2-phosphoglycerate using 2,3-bisphosphoglycerate as a cofactor. Also mediates the interconversion of (2R)-2,3-bisphosphoglycerate and (2R)-3-phospho-glyceroyl phosphate. Related references: PMID:23653202
• Highly expressed in the liver and brain but not detected in muscle, suggesting tissue-specific roles in glycolysis and energy metabolism.
• Subject to post-translational modifications, including acetylation and phosphorylation, which may regulate its enzymatic activity and interactions.
• The calculated molecular weight is approximately 28.8 kDa, migrating at around 29 kDa under denaturing conditions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–100), so the antibody likely recognizes an epitope within this domain. Confirm recognition of full-length endogenous protein in relevant sample systems.
• For Western blotting, use liver or brain lysates as positive controls; expect a single band at ~29 kDa. Optimize loading amounts and antibody dilution for your specific experimental conditions.
• For immunohistochemistry (IHC-P), standard antigen retrieval may be necessary; validate staining patterns in human liver or brain tissue sections and include appropriate negative controls.
• Cross-reactivity with mouse and rat has been reported, but always verify antibody performance in the species and application of interest before large-scale use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a streamlined procurement platform for IVD raw materials, connecting diagnostic manufacturers and researchers with high-quality reagents like this anti-PGAM1 monoclonal antibody. Targeting the glycolytic enzyme PGAM1, this antibody supports research in cancer metabolism, metabolic disorders, and related diagnostic applications. Alongside validated antibody pairs and optimized monoclonal/polyclonal antibodies, we offer bulk ancillary reagents and custom sourcing for rare targets to accelerate your assay development from concept to clinic.