Anti-PGA3 Rabbit Polyclonal Antibody for WB, ELISA - P0DJD8

Core Product Specifications and Parameters

Biological Background: PGA3 Function and Localization

• PGA3, also known as Pepsinogen-3 or Pepsin A-3, is an aspartyl protease belonging to the pepsin family of digestive enzymes.
• The protein is produced as an inactive zymogen (pepsinogen) that requires proteolytic cleavage for activation, a process involving signal peptide removal and autocatalytic processing.
• PGA3 exhibits broad substrate specificity, preferentially cleaving peptide bonds involving phenylalanine and leucine residues, with additional activity on various other sites.
• As a secreted enzyme, PGA3 functions extracellularly in the digestive tract, contributing to protein digestion.
• The enzyme is characterized by an active site containing two aspartic acid residues, essential for its catalytic mechanism as an aspartyl protease.
• Structural features include disulfide bonds that stabilize its three-dimensional conformation.
• PGA3 has been identified in proteomic analyses, and its sequence has been directly verified at the protein level (direct protein sequencing evidence).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 63–180 of human PGA3, which spans part of the pepsinogen sequence; the antibody may recognize both the zymogen and active pepsin forms.
• For Western blot (WB), consider testing both reducing and non-reducing conditions due to disulfide bonds that may affect migration.
• Based on the predicted molecular weight of 42 kDa, confirm the band size in your experimental system, accounting for potential glycosylation or processing.
• For ELISA, validate the antibody against recombinant PGA3 or native pepsinogen to ensure specificity.
• Given cross-reactivity with mouse and rat, verify suitability in those species by aligning target sequences.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to critical IVD raw materials like this anti-PGA3 antibody. As a one-stop sourcing bridge, we offer validated antibody pairs, bulk polyclonal and monoclonal antibodies, and ancillary reagents for digestive enzyme and protease research. Our supply chain expertise supports the development of robust immunoassays from concept to clinic.