Anti-PFN2 Rabbit Polyclonal Antibody for WB, ELISA - P35080

Core Product Specifications and Parameters

Biological Background: Profilin-2 Function and Localization

• Profilin-2 (PFN2), also known as Profilin II, is a member of the profilin family of actin-binding proteins.
• It binds to monomeric actin (G-actin) and regulates actin polymerization dynamics, promoting polymerization at low concentrations and inhibiting at high concentrations.
• Profilin-2 also interacts with phosphatidylinositol 4,5-bisphosphate (PIP2), inhibiting the hydrolysis of PIP2 into inositol triphosphate (IP3) and diacylglycerol (DG), thus modulating intracellular signaling.
• The protein localizes predominantly in the cytoplasm and associates with the cytoskeleton.
• PFN2 exhibits tissue-specific expression, with high levels in brain, skeletal muscle, and kidney; moderate expression in heart, placenta, lung, and liver.
• Post-translational modifications include acetylation, and the protein undergoes alternative splicing.
• Profilin-2 plays a critical role in cytoskeleton remodeling and actin-mediated processes such as cell motility and intracellular transport.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide spanning amino acids 100-140 of human PFN2. Ensure that the target epitope is conserved in your species of interest.
• For western blotting, the expected band size is approximately 15 kDa. Optimize loading controls accordingly.
• When performing ELISA, consider using a matched antibody pair for sandwich assays, and validate detection limits in your sample matrix.
• Cross-reactivity is predicted with mouse and rat PFN2; however, verify specificity in relevant tissues due to potential isoform expression.
• Given profilin-2's involvement in actin dynamics, cellular extraction and lysis buffers should be chosen carefully to preserve protein complexes if studying interactions.
• To reduce non-specific binding, pre-absorb the antibody against the immunogen or use appropriate blocking agents.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials. From validated antibody pairs and recombinant proteins to bulk ancillary reagents, we streamline your procurement for targets like Profilin-2. Our team can also assist with sourcing rare raw materials and technical consulting to accelerate your assay development.