Anti-PFN2 Polyclonal Antibody for WB, ELISA - P35080

Core Product Specifications and Parameters

Biological Background: Profilin-2 Function and Localization

• Profilin-2 (PFN2), also known as Profilin II, is an actin-binding protein that modulates cytoskeletal dynamics by regulating actin polymerization in a concentration-dependent manner.
• At low concentrations, profilin-2 promotes actin polymerization, while at high concentrations it sequesters actin monomers and inhibits filament formation.
• PFN2 binds phosphatidylinositol 4,5-bisphosphate (PIP2), thereby inhibiting the hydrolysis of PIP2 to inositol trisphosphate (IP3) and diacylglycerol (DG), which links actin dynamics to phosphoinositide signaling.
• The protein is localized to the cytoplasm and associates with the cytoskeleton, consistent with its role in actin remodeling.
• PFN2 is highly expressed in brain, skeletal muscle, and kidney, with lower levels in heart, placenta, lung, and liver, suggesting tissue-specific functions in motility and structural organization.
• Alternative splicing of the PFN2 gene generates multiple transcript variants, contributing to isoform diversity (keyword: Alternative splicing).
• Post-translational modifications include acetylation (keyword: Acetylation), which may regulate protein stability or interactions.
• Profilin-2 is subject to direct protein sequencing and proteomics identification, validating its endogenous expression (keyword: Direct protein sequencing, Proteomics identification).

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein covering amino acids 1-140 of human PFN2 (NP_444252.1). Consider that the antibody may detect both full-length profilin-2 and N-terminal fragments.
• For western blotting, PFN2 has a predicted molecular weight of approximately 15 kDa; we recommend using appropriate gel percentages and transfer conditions for small proteins.
• Due to the cytoplasmic localization, consider preparing whole-cell lysates with suitable buffers, and validate expression in cell lines or tissues with known PFN2 abundance (e.g., brain, skeletal muscle).
• Cross-reactivity is indicated for human, mouse, and rat; however, we recommend verifying sequence homology for other species if needed.
• ELISA applications may require optimization of coating conditions and detection antibodies; consider pairing with a validated secondary antibody and appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines procurement for diagnostic manufacturers and research laboratories by providing validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents covering the full IVD raw-material spectrum. For PFN2-related projects, we offer polyclonal antibodies suitable for cytoskeleton and actin dynamics research, along with rare target raw-material sourcing. We are your one-stop bridge from concept to clinic, ensuring reliable supply chains for IVD development.