Anti-PFKP Rabbit Polyclonal Antibody for WB and ELISA - Q01813

Core Product Specifications and Parameters

Biological Background: PFKP Function and Localization

• PFKP is the platelet-type isoform of phosphofructokinase (PFK), a key regulatory enzyme in glycolysis.
• It catalyzes the ATP-dependent phosphorylation of fructose 6-phosphate to fructose 1,6-bisphosphate, the first irreversible step specific to glycolysis.
• The enzyme functions as an allosteric enzyme, regulated by various metabolites, allowing precise control of glycolytic flux.
• PFKP localizes primarily to the cytoplasm, consistent with its role in the glycolytic pathway.
• Post-translational modifications include acetylation and phosphorylation, which may modulate enzyme activity and stability.
• The protein is subject to alternative splicing, potentially generating isoforms with distinct regulatory properties.
• PFKP is a glycoprotein and binds magnesium as a cofactor, essential for catalytic activity.
• It belongs to the phosphofructokinase family and contains nucleotide-binding domains for ATP.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 605-784), which is relatively conserved; validate specificity in your sample system.
• For WB, a band at approximately 86 kDa is expected; optimal dilution should be determined experimentally.
• ELISA conditions may require optimization; consider testing a range of coating antigen concentrations and antibody dilutions.
• This antibody is validated for human samples; cross-reactivity with other species has not been tested and should be verified.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials, including validated antibodies like this anti-PFKP polyclonal antibody for glycolysis and cancer metabolism research. Our platform supports diagnostic manufacturers and labs with monoclonal/polyclonal antibodies, bulk reagents, and rare target sourcing, streamlining your development from concept to clinic.